To develop and evaluate primer sets targeted to the dissimilatory sulfite reductase gene (dsrA) for use in quantitative real-time PCR detection of sulfate-reducing bacteria (SRB) in stored swine manure.
Development and comparison of SYBR Green quantitative real-time PCR assays for detection and enumeration of sulfate-reducing bacteria in stored swine manure.
Fermentation Biotechnology Research Unit, National Center for Agricultural Research, USDA, Agricultural Research Service, Peoria, IL 61604, USA. cheryl.spence@ars.usda.gov