| pubmed-article:1911883 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1911883 | lifeskim:mentions | umls-concept:C0035647 | lld:lifeskim |
| pubmed-article:1911883 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:1911883 | lifeskim:mentions | umls-concept:C1257739 | lld:lifeskim |
| pubmed-article:1911883 | lifeskim:mentions | umls-concept:C1522484 | lld:lifeskim |
| pubmed-article:1911883 | lifeskim:mentions | umls-concept:C0036525 | lld:lifeskim |
| pubmed-article:1911883 | lifeskim:mentions | umls-concept:C0033713 | lld:lifeskim |
| pubmed-article:1911883 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:1911883 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
| pubmed-article:1911883 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1911883 | pubmed:dateCreated | 1991-11-13 | lld:pubmed |
| pubmed-article:1911883 | pubmed:abstractText | A plasmid containing the K-fgf proto-oncogene linked to the dihydrofolate reductase gene has been constructed, and used in transfection experiments to investigate the effects of K-fgf expression on the tumorigenic and metastatic properties of NIH-3T3 fibroblasts. Analysis of cells transfected with K-fgf revealed that expression of the K-fgf proto-oncogene can, in a single step, induce both tumorigenic and metastatic characteristics, as determined in soft agar cloning experiments, and in tumorigenicity and experimental lung metastasis assays with BALB/c nu/nu mice. Selection for resistance to increasing concentrations of methotrexate lead to the isolation of a series of cell lines containing amplifications of both the dihydrofolate reductase gene and the linked K-fgf gene, which synthesized elevated levels of growth factor message and protein. The most highly resistant and gene amplified cell lines exhibited lower than expected levels of K-fgf mRNA, and also appeared to have down-regulated cell surface growth factor receptors. Further support for the concept that altered K-fgf expression can induce fully malignant and metastatic cells was obtained in experimental metastasis assays, where K-fgf transfected and gene amplified cell lines were highly aggressive. | lld:pubmed |
| pubmed-article:1911883 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1911883 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1911883 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1911883 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1911883 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:1911883 | pubmed:author | pubmed-author:WrightJ AJA | lld:pubmed |
| pubmed-article:1911883 | pubmed:author | pubmed-author:GreenbergA... | lld:pubmed |
| pubmed-article:1911883 | pubmed:author | pubmed-author:DamenJ EJE | lld:pubmed |
| pubmed-article:1911883 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1911883 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:1911883 | pubmed:volume | 1097 | lld:pubmed |
| pubmed-article:1911883 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1911883 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1911883 | pubmed:pagination | 103-10 | lld:pubmed |
| pubmed-article:1911883 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:1911883 | pubmed:meshHeading | pubmed-meshheading:1911883-... | lld:pubmed |
| pubmed-article:1911883 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1911883 | pubmed:articleTitle | Transformation and amplification of the K-fgf proto-oncogene in NIH-3T3 cells, and induction of metastatic potential. | lld:pubmed |
| pubmed-article:1911883 | pubmed:affiliation | Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada. | lld:pubmed |
| pubmed-article:1911883 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1911883 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
