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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-11-13
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pubmed:abstractText |
A plasmid containing the K-fgf proto-oncogene linked to the dihydrofolate reductase gene has been constructed, and used in transfection experiments to investigate the effects of K-fgf expression on the tumorigenic and metastatic properties of NIH-3T3 fibroblasts. Analysis of cells transfected with K-fgf revealed that expression of the K-fgf proto-oncogene can, in a single step, induce both tumorigenic and metastatic characteristics, as determined in soft agar cloning experiments, and in tumorigenicity and experimental lung metastasis assays with BALB/c nu/nu mice. Selection for resistance to increasing concentrations of methotrexate lead to the isolation of a series of cell lines containing amplifications of both the dihydrofolate reductase gene and the linked K-fgf gene, which synthesized elevated levels of growth factor message and protein. The most highly resistant and gene amplified cell lines exhibited lower than expected levels of K-fgf mRNA, and also appeared to have down-regulated cell surface growth factor receptors. Further support for the concept that altered K-fgf expression can induce fully malignant and metastatic cells was obtained in experimental metastasis assays, where K-fgf transfected and gene amplified cell lines were highly aggressive.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/FGF4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fgf4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Methotrexate,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrahydrofolate Dehydrogenase
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
1097
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-10
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1911883-Animals,
pubmed-meshheading:1911883-Cell Line,
pubmed-meshheading:1911883-Cell Transformation, Neoplastic,
pubmed-meshheading:1911883-Drug Resistance,
pubmed-meshheading:1911883-Fibroblast Growth Factor 2,
pubmed-meshheading:1911883-Fibroblast Growth Factor 4,
pubmed-meshheading:1911883-Fibroblast Growth Factors,
pubmed-meshheading:1911883-Gene Amplification,
pubmed-meshheading:1911883-Growth Substances,
pubmed-meshheading:1911883-Humans,
pubmed-meshheading:1911883-Methotrexate,
pubmed-meshheading:1911883-Mice,
pubmed-meshheading:1911883-Mice, Inbred BALB C,
pubmed-meshheading:1911883-Mice, Nude,
pubmed-meshheading:1911883-Neoplasm Metastasis,
pubmed-meshheading:1911883-Plasmids,
pubmed-meshheading:1911883-Proto-Oncogene Proteins,
pubmed-meshheading:1911883-Proto-Oncogenes,
pubmed-meshheading:1911883-Radioligand Assay,
pubmed-meshheading:1911883-Tetrahydrofolate Dehydrogenase,
pubmed-meshheading:1911883-Transfection,
pubmed-meshheading:1911883-Tumor Cells, Cultured,
pubmed-meshheading:1911883-Tumor Stem Cell Assay
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pubmed:year |
1991
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pubmed:articleTitle |
Transformation and amplification of the K-fgf proto-oncogene in NIH-3T3 cells, and induction of metastatic potential.
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pubmed:affiliation |
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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