Linked Life Data

19118514

Source:http://linkedlifedata.com/resource/pubmed/id/19118514

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-1-2
pubmed:abstractText
The wild-type human Fas-associated death domain (FADD) protein was expressed as a His-tag fusion protein in Escherichia coli. Recombinant FADD proteins were purified under the denatured condition. After denatured protein purification, it was refolded and obtained at a yield of about 23 mg/L. Purified FADD exhibited as a homogenous band corresponding to the molecular weight of 31 kDa. Immunization of rabbits against the refolded FADD protein was allowed the production of high titre polyclonal antiserum. This new polyclonal antibody could recognize recombinant FADD protein in Western blot. Immunoreactivity was also observed in immunofluorescence assay. The low cost polyclonal antiserum was applicable to extensive detection of FADD in various immunoassays.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
2042-0226
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
471-4
pubmed:dateRevised
2010-3-15
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Expression of recombinant human FADD, preparation of its polyclonal antiserum and the application in immunoassays.
pubmed:affiliation
The State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Center of Hepatobiliary Diseases, Nanjing University, Nanjing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't