Linked Life Data

19116690

Source:http://linkedlifedata.com/resource/pubmed/id/19116690

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-2-27
pubmed:abstractText
Semliki forest virus (SFV) is a pathogen causing lethal encephalitis in laboratory mice. In this study, we obtained three short hairpin RNAs (shRNAs) which could specifically target SFV sequence in GFP reporting systems and effectively suppress SFV replication in luciferase-containing reporter virus system. At a multiplicity of infection (MOI) of 0.001, the luciferase reporter activity was reduced by 78-92% by shRNA expression plasmids and virus yields reduced 2 to 10-fold at 20 h post-infection. When lentiviral vector-derived shRNAs were employed, the virus titers decreased 8 to 126-fold at 24 h post-infection and 6 to 19-fold at 48 h post-infection and the cell survival was prolonged. These data formed the basis for further in vivo studies of RNA interference in mouse models.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1573-6776
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
501-8
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Lentiviral vector-derived shRNAs confer enhanced suppression of Semliki forest virus replication in BHK-21 cells compared to shRNAs expressed from plasmids.
pubmed:affiliation
State Key Laboratory of Virology, The Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't