Linked Life Data

19114520

Source:http://linkedlifedata.com/resource/pubmed/id/19114520

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-2-26
pubmed:abstractText
Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilms produced in vitro by the gram-negative porcine respiratory pathogen Actinobacillus pleuropneumoniae and the gram-positive device-associated pathogen Staphylococcus epidermidis. The effect of PNAG on bulk fluid flow was determined by measuring the rate of fluid convection through biofilms cultured in centrifugal filter devices. The rate of fluid convection was significantly higher in biofilms cultured in the presence of the PNAG-degrading enzyme dispersin B than in biofilms cultured without the enzyme, indicating that PNAG decreases bulk fluid flow. PNAG also blocked transport of the quaternary ammonium compound cetylpyridinium chloride (CPC) through the biofilms. Binding of CPC to biofilms further impeded fluid convection and blocked transport of the azo dye Allura red. Bioactive CPC was efficiently eluted from biofilms by treatment with 1 M sodium chloride. Taken together, these findings suggest that CPC reacts directly with the PNAG matrix and alters its physical and chemical properties. Our results indicate that PNAG plays an important role in controlling the physiological state of biofilms and may contribute to additional biofilm-associated processes such as biocide resistance.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-10496925, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-11166241, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-11336839, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-11850284, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-11959584, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-12591863, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-12896987, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-13059024, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-1314224, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-14764110, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-15215120, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-15501828, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-15576769, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-15601723, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-15639630, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-16177366, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-16321928, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-17114249, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-17412552, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-17586629, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-17600053, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-17851029, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-18515487, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-18573331, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-2295856, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-8039894, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-8109913, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-8941774, http://linkedlifedata.com/resource/pubmed/commentcorrection/19114520-9880479
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1098-5336
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1308-14
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Poly-N-acetylglucosamine matrix polysaccharide impedes fluid convection and transport of the cationic surfactant cetylpyridinium chloride through bacterial biofilms.
pubmed:affiliation
Department of Oral Biology, New Jersey Dental School, Newark, New Jersey, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural