Source:http://linkedlifedata.com/resource/pubmed/id/19109951
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2009-2-16
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| pubmed:abstractText |
We and others have reported that neural stem/progenitor cells (NSCs) may exert direct anti-inflammatory activity. This action has been attributed, in part, to T-cell suppression. However, how T-cells become suppressed by NSCs remains unresolved. In this study, we explored one of these mechanisms and challenged some previously advanced hypotheses regarding underlying NSC-mediated T-cell suppression. We employed an easily observable and manipulatable system in which activated and non-activated T-cells were co-cultured with a stable well-characterized clone of lacZ-expressing murine NSCs. As in previous reports, NSCs were found to inhibit T-cell proliferation. However, this inhibition by NSCs was not due to suppression of T cell activation or induction of apoptosis of T cells during the early activation stage. High levels of nitric oxide (NO) and prostaglandin E2 (PGE2) were induced in the T cells when co-cultured with NSCs. In addition, inducible NOS (iNOS) and microsomal type 1 PGES (mPGES-1) were readily detected in NSCs in co-culture with T-cells, but not at all in NSCs cultured alone or in activated T cells cultured with or without NSCs. This finding suggested that activated T cells induced NO and PGE2 production in the NSCs. Furthermore, T-cell proliferation inhibited by co-culture with the NSCs was significantly restored by inhibitors of NO and PGE2 production. Therefore, NSCs appear to suppress T-cells, at least in part, by NO and PGE2 production which, in turn, would account for the well-documented reduction of central nervous system immunopathology by transplanted NSCs.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Intramolecular Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/prostaglandin-E synthase
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1090-2430
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
216
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
177-83
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| pubmed:meshHeading |
pubmed-meshheading:19109951-Animals,
pubmed-meshheading:19109951-Cell Communication,
pubmed-meshheading:19109951-Cell Proliferation,
pubmed-meshheading:19109951-Cells, Cultured,
pubmed-meshheading:19109951-Central Nervous System,
pubmed-meshheading:19109951-Coculture Techniques,
pubmed-meshheading:19109951-Dinoprostone,
pubmed-meshheading:19109951-Encephalitis,
pubmed-meshheading:19109951-Enzyme Inhibitors,
pubmed-meshheading:19109951-Genes, Reporter,
pubmed-meshheading:19109951-Immune Tolerance,
pubmed-meshheading:19109951-Immunity, Cellular,
pubmed-meshheading:19109951-Intramolecular Oxidoreductases,
pubmed-meshheading:19109951-Lac Operon,
pubmed-meshheading:19109951-Mice,
pubmed-meshheading:19109951-Mice, Inbred C57BL,
pubmed-meshheading:19109951-Nitric Oxide,
pubmed-meshheading:19109951-Nitric Oxide Synthase Type II,
pubmed-meshheading:19109951-Stem Cell Transplantation,
pubmed-meshheading:19109951-Stem Cells,
pubmed-meshheading:19109951-T-Lymphocytes,
pubmed-meshheading:19109951-Up-Regulation
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| pubmed:year |
2009
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| pubmed:articleTitle |
Neural stem/progenitor cells modulate immune responses by suppressing T lymphocytes with nitric oxide and prostaglandin E2.
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| pubmed:affiliation |
Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5525, USA. leiwang@vet.k-state.edu
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| pubmed:publicationType |
Journal Article
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