Linked Life Data

19109409

Source:http://linkedlifedata.com/resource/pubmed/id/19109409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-5-1
pubmed:abstractText
Although advanced age is linked with an increased incidence of lung carcinomas, elderly patients develop fewer metastatic tumors than their younger counterparts. Since extracellular matrix controls carcinogenesis, we studied the effect of aged fibrillar collagen (rat tail) on the invasiveness of lung carcinoma cells (H322, H358). Two- and three-dimensional invasion assays revealed a reduced migration of lung cancer cells through a matrix of collagen from old (24 mo) compared with young (2 mo) or adult (12 mo) rats. This was most strongly observed for H322 cells, which had a better migration behavior than H358 cells. Since old collagen was increasingly modified by advanced glycation end products (AGEs; fluorescing AGEs, nonfluorescing carboxymethyllysine), we generated different AGE-collagens by treating collagen with ribose or alpha-dicarbonyls. Similarly to old collagen, AGE-collagen reduced the invasive migration of H322 cells. Subsequent analyses demonstrated that old and AGE-collagens impair distinct mechanisms contributing to cell migration, i.e., efficient cell adhesion and proteolytic degradation of collagen by membrane-type matrix metalloproteinase (MT-MMP; MT-MMP3 in H322 cells). Our data indicate that age-related alterations of the extracellular matrix contribute to a less invasive behavior of lung carcinoma cells, in which AGE modifications of aged collagen seem to play an important role.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1530-6860
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1510-20
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Age-associated changes of extracellular matrix collagen impair lung cancer cell migration.
pubmed:affiliation
Department of Cardio-thoracic Surgery, University Hospital Halle (Saale), Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany. babett.bartling@medizin.uni-halle.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't