Source:http://linkedlifedata.com/resource/pubmed/id/19105227
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2009-6-15
|
| pubmed:abstractText |
We examined local and systemic antiinflammatory consequences of ischemic preconditioning (IPC) in a rat model of limb ischemia-reperfusion (I-R) by characterizing the leukocyte-endothelial interactions in the periosteum and the expression of adhesion molecules playing a role in leukocyte-mediated inflammatory processes. IPC induction (2 cycles of 10 min of complete limb ischemia and 10 min of reperfusion) was followed by 60 min of ischemia/180 min of reperfusion or sham-operation. Data were compared with those on animals subjected to I-R and sham-operation. Neutrophil leukocyte-endothelial cell interactions (intravital videomicroscopy), intravascular neutrophil activation (CD11b expression changes by flow cytometry), and soluble and tissue intercellular adhesion molecule-1 (ICAM-1; ELISA and immunohistochemistry, respectively) expressions were assessed. I-R induced enhanced leukocyte rolling and adherence in the periosteal postcapillary venules after 120 and 180 min of reperfusion. This was associated with a significantly enhanced CD11b expression (by approximately 80% and 72%, respectively) and moderately increased soluble and periosteal ICAM-1 expressions. IPC prevented the I-R-induced increases in leukocyte adherence and CD11b expression without influencing the soluble and tissue ICAM-1 levels. The results show that limb IPC exerts not only local, but distant antiinflammatory effects through significant modulation of neutrophil recruitment.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
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| pubmed:issn |
1554-527X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
27
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
897-902
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| pubmed:meshHeading |
pubmed-meshheading:19105227-Animals,
pubmed-meshheading:19105227-Antigens, CD11b,
pubmed-meshheading:19105227-Down-Regulation,
pubmed-meshheading:19105227-Hindlimb,
pubmed-meshheading:19105227-Immunohistochemistry,
pubmed-meshheading:19105227-Intercellular Adhesion Molecule-1,
pubmed-meshheading:19105227-Ischemic Preconditioning,
pubmed-meshheading:19105227-Leukocyte Rolling,
pubmed-meshheading:19105227-Male,
pubmed-meshheading:19105227-Microcirculation,
pubmed-meshheading:19105227-Microscopy, Video,
pubmed-meshheading:19105227-Neutrophils,
pubmed-meshheading:19105227-Periosteum,
pubmed-meshheading:19105227-Rats,
pubmed-meshheading:19105227-Rats, Sprague-Dawley,
pubmed-meshheading:19105227-Regional Blood Flow,
pubmed-meshheading:19105227-Reperfusion Injury,
pubmed-meshheading:19105227-Tibia,
pubmed-meshheading:19105227-Venules
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Ischemic limb preconditioning downregulates systemic inflammatory activation.
|
| pubmed:affiliation |
Institute of Surgical Research, University of Szeged, H-6720 Szeged, Pécsi u. 6, Hungary. sza@expsur.szote.u-szeged.hu
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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