Linked Life Data

19104482

Source:http://linkedlifedata.com/resource/pubmed/id/19104482

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-23
pubmed:abstractText
This study found that in Tris-HCl buffer, the resonance light scattering (RLS) intensity of the Eu(3+)-nicotinic acid system can be greatly enhanced by nucleic acids and the enhanced intensity is proportional to the concentration of nucleic acid in the range of 7 x 10(-8)-1 x 10(-5 ) g x mL(-1) for fsDNA, and its detection limit is 2 x 10(-8 ) g x mL(-1). Based on this, a new method for the determination of nucleic acids is proposed. Synthetic and actual samples are determined satisfactorily. The interaction mechanism is also studied. It is thought that nucleic acid can bind with the Eu(3+)-nicotinic acid complex through electrostatic attraction and thus form a large Eu(3+)-nicotinic acid-nucleic acid complex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1420-3049
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-8
pubmed:dateRevised
2009-10-27
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
A new method for the determination of nucleic acid using an Eu3+- nicotinic acid complex as a resonance light scattering probe.
pubmed:affiliation
Microscale Science Institute, Department of Chemistry, Weifang University, Weifang 261061, PR China. guomeng7101@126.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't