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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2008-12-30
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pubmed:abstractText |
Poly-ADP ribose polymerase 1 (PARP-1) is activated by DNA damage and has been implicated in the repair of single-strand breaks (SSBs). Involvement of PARP-1 in other DNA damage responses remains controversial. In this study, we show that PARP-1 is required for replication fork slowing on damaged DNA. Fork progression in PARP-1(-/-) DT40 cells is not slowed down even in the presence of DNA damage induced by the topoisomerase I inhibitor camptothecin (CPT). Mammalian cells treated with a PARP inhibitor or PARP-1-specific small interfering RNAs show similar results. The expression of human PARP-1 restores fork slowing in PARP-1(-/-) DT40 cells. PARP-1 affects SSB repair, homologous recombination (HR), and nonhomologous end joining; therefore, we analyzed the effect of CPT on DT40 clones deficient in these pathways. We find that fork slowing is correlated with the proficiency of HR-mediated repair. Our data support the presence of a novel checkpoint pathway in which the initiation of HR but not DNA damage delays the fork progression.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-10400681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-10467406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-10747037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-11139322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-11292338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-12718895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-12897160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-12930846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-13679147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-14617623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-14643436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-14734561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-14762204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-15247263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-15280503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-15367657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-15657438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-15744309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-16278671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-16452972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-16498404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-17589503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-2562007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-3571334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-8948628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-9278517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-9518481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-9584196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19103807-9736627
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1540-8140
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
29
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pubmed:volume |
183
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1203-12
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:19103807-Animals,
pubmed-meshheading:19103807-COS Cells,
pubmed-meshheading:19103807-Camptothecin,
pubmed-meshheading:19103807-Cells, Cultured,
pubmed-meshheading:19103807-Cercopithecus aethiops,
pubmed-meshheading:19103807-DNA Breaks, Double-Stranded,
pubmed-meshheading:19103807-DNA Damage,
pubmed-meshheading:19103807-DNA Replication,
pubmed-meshheading:19103807-HeLa Cells,
pubmed-meshheading:19103807-Humans,
pubmed-meshheading:19103807-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:19103807-Recombination, Genetic
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pubmed:year |
2008
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pubmed:articleTitle |
PARP-1 ensures regulation of replication fork progression by homologous recombination on damaged DNA.
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pubmed:affiliation |
Department of Life Science, Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan. sugimura@bio.mie-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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