Linked Life Data

19103154

Source:http://linkedlifedata.com/resource/pubmed/id/19103154

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-1-21
pubmed:abstractText
Histamine often causes inflammation, and this amine is produced by histidine decarboxylase (HDC). We found that (-)-DHMEQ, an NF-kappaB inhibitor, inhibited lipopolysaccharide (LPS)-induced histamine production and HDC induction in mouse macrophage cell line RAW264.7. However, as there is no kappaB site in the HDC promoter, we studied the mechanism of inhibition. Knockdown of the transcription factor C/EBPbeta reduced the HDC expression in LPS-treated cells. (-)-DHMEQ inhibited the C/EBPbeta transcriptional activity in a reporter assay and in an electrophoresis mobility shift assay. But it did not inhibit the in vitro binding of C/EBPbeta to DNA. It also did not lower the nuclear amount of C/EBPbeta. On the other hand, the addition of recombinant p65, a component of NF-kappaB, enhanced the activity of C/EBPbeta acting as a cofactor in vitro. Then, we found that (-)-DHMEQ lowered the nuclear amount of p65. Thus, inhibition of the C/EBPbeta activity by (-)-DHMEQ would be due to a reduction in the amount of nuclear p65, which has a co-activator activity for C/EBPbeta that is essential for the HDC induction. (-)-DHMEQ may be useful as an anti-inflammatory agent by lowering the histamine production in the body.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1090-2104
pubmed:author
pubmed:issnType
Electronic
pubmed:day
6
pubmed:volume
379
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
379-83
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Induction of histidine decarboxylase in macrophages inhibited by the novel NF-kappaB inhibitor (-)-DHMEQ.
pubmed:affiliation
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-0061, Japan. eriboeribo@hotmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't