Linked Life Data

19097900

Source:http://linkedlifedata.com/resource/pubmed/id/19097900

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2008-12-25
pubmed:abstractText
Most plant organs develop postembryonically from stem cells in the shoot and root meristems. In Arabidopsis, Class I KNOTTED-like homeobox (KNOX) transcription factors are specifically expressed in shoot meristems and play a primary role in the maintenance of meristem function. Although suppression of KNOX was shown to associate with histone H3K27-methylation, the molecular mechanism underlying this suppression is not well understood. Here, we provide genetic, molecular, and functional evidence that an Arabidopsis POLYCOMB REPRESSIVE COMPLEX1 (PRC1)-like complex acts in conjunction with PRC2 in KNOX suppression. We identified AtRING1a and AtRING1b as homologs of the animal PRC1 core component RING1. Loss-of-function mutant Atring1a(-/-)Atring1b(-/-) shows release of KNOX suppression and ectopic-meristem formation. AtRING1a and AtRING1b proteins are localized in the nucleus. AtRING1a binds to itself and to AtRING1b, to CURLY LEAF (CLF), a PRC2 core component catalyzing H3K27-methylation, and to LIKE HETEROCHROMATIN PROTEIN1 (LHP1), a chromodomain protein binding trimethyl-H3K27. We further show that clf(-/-) and lhp1(-/-) enhance Atring1a(-/-)Atring1b(-/-) in release of KNOX suppression and mutant phenotypes. We propose a model in which AtRING1a, AtRING1b, and LHP1 form a PRC1-like complex, which binds trimethyl-H3K27 marked by the CLF-containing PRC2, resulting in transcriptional suppression of KNOX.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1879-0445
pubmed:author
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1966-71
pubmed:dateRevised
2009-4-29
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Polycomb silencing of KNOX genes confines shoot stem cell niches in Arabidopsis.
pubmed:affiliation
Institut de Biologie Moléculaire des Plantes (IBMP), Centre National de la Recherche Scientifique (CNRS), Université Louis Pasteur de Strasbourg (ULP), Strasbourg Cédex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't