Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
52
pubmed:dateCreated
2008-12-31
pubmed:abstractText
The selectin family of leukocyte adhesion receptors is principally recognized for mediating transient rolling interactions during the inflammatory response. Recent studies using ultrasensitive force probes to characterize the force-lifetime relationship between P- and L-selectin and their endogenous ligands have underscored the ability of increasing levels of force to initially extend the lifetime of these complexes before disrupting bond integrity. This so-called "catch-slip" transition has provided an appealing explanation for shear threshold phenomena in which increasing levels of shear stress stabilize leukocyte rolling under flow. We recently incorporated catch-slip kinetics into a mechanical model for cell adhesion and corroborated this hypothesis for neutrophils adhering via L-selectin. Here, using adhesive dynamics simulations, we demonstrate that biomembrane force probe measurements of various P- and L-selectin catch bonds faithfully predict differences in cell adhesion patterns that have been described extensively in vitro. Using phenomenological parameters to characterize the dominant features of molecular force spectra, we construct a generalized phase map that reveals that robust shear-threshold behavior is possible only when an applied force very efficiently stabilizes the bound receptor complex. This criteria explains why only a subset of selectin catch bonds exhibit a shear threshold and leads to a quantitative relationship that may be used to predict the magnitude of the shear threshold for families of catch-slip bonds directly from their force spectra. Collectively, our results extend the conceptual framework of adhesive dynamics as a means to translate complex single-molecule biophysics to macroscopic cell behavior.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-10049312, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-10570148, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-11005837, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-11081633, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-11158576, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-11423392, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-11566773, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-11907045, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-12324413, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-12736689, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-1384734, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-14573602, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-14597772, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-15277675, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-15364963, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-15701706, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-15879471, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-15951391, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-16258054, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-16272438, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-16624825, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-16845394, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-17000883, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-17028146, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-17085490, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-17181300, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-17890384, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-18299992, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-18573088, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-347575, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-7535385, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-8538793, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-8626430, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-8896391, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-9024700, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-9281593, http://linkedlifedata.com/resource/pubmed/commentcorrection/19095798-9618492
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
30
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
20716-21
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Selectin catch-slip kinetics encode shear threshold adhesive behavior of rolling leukocytes.
pubmed:affiliation
Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural