Source:http://linkedlifedata.com/resource/pubmed/id/19095055
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2009-3-2
|
| pubmed:abstractText |
Actinobacillus pleuropneumoniae is the causative agent of porcine contagious pleuropneumonia, a very important swine respiratory infectious disease causing great economic losses worldwide. The pathogenesis of this disease is still not completely understood. Biofilm formation contributes to full virulence in many Gram-negative bacterial pathogens. In the present study, two biofilm-producing mutants were identified from the transposon mutagenesis mutant pools of A. pleuropneumoniae strain 4074 of serovar 1 (a non-biofilm forming strain). Inverse PCR and sequencing analysis revealed that the hns gene encoding the histone-like nucleoid structuring protein (H-NS) was inactivated by the mini-Tn10 transposon in both mutant strains. Further analysis revealed that the virulence was attenuated in the mutant strains when their haemolytic activity and 50% lethal doses in mice were compared with the parental strain. Real-time RT-PCR analysis suggested that the down-regulation of the exotoxin genes in the hns mutants may partly contribute to the virulence attenuation. Our data indicate that H-NS plays important roles in regulating biofilm formation and virulence in A. pleuropneumoniae.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Transposable Elements,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/H-NS protein, bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0882-4010
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
128-34
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| pubmed:meshHeading |
pubmed-meshheading:19095055-Actinobacillus Infections,
pubmed-meshheading:19095055-Actinobacillus pleuropneumoniae,
pubmed-meshheading:19095055-Animals,
pubmed-meshheading:19095055-Bacterial Proteins,
pubmed-meshheading:19095055-Bacterial Toxins,
pubmed-meshheading:19095055-Biofilms,
pubmed-meshheading:19095055-DNA Transposable Elements,
pubmed-meshheading:19095055-DNA-Binding Proteins,
pubmed-meshheading:19095055-Female,
pubmed-meshheading:19095055-Gene Deletion,
pubmed-meshheading:19095055-Gene Expression Regulation, Bacterial,
pubmed-meshheading:19095055-Hemolysis,
pubmed-meshheading:19095055-Lethal Dose 50,
pubmed-meshheading:19095055-Mice,
pubmed-meshheading:19095055-Mice, Inbred BALB C,
pubmed-meshheading:19095055-Mutagenesis, Insertional,
pubmed-meshheading:19095055-Virulence,
pubmed-meshheading:19095055-Virulence Factors
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| pubmed:year |
2009
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| pubmed:articleTitle |
Histone-like protein H-NS regulates biofilm formation and virulence of Actinobacillus pleuropneumoniae.
|
| pubmed:affiliation |
Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan 430070, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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