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rdf:type |
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lifeskim:mentions |
umls-concept:C0026237,
umls-concept:C0127400,
umls-concept:C0205263,
umls-concept:C0205409,
umls-concept:C0596235,
umls-concept:C0596311,
umls-concept:C0597484,
umls-concept:C0878412,
umls-concept:C1120861,
umls-concept:C1330957,
umls-concept:C1621574,
umls-concept:C1948023
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pubmed:issue |
1-2
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pubmed:dateCreated |
2009-2-3
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pubmed:abstractText |
Treatment of bovine pulmonary artery smooth muscle mitochondria with the calcium ionophore, A23187 (0.2 microM) stimulates mu-calpain activity and subsequently cleaves Na(+)/Ca(2+) exchanger (NCX). Pretreatment of the A23187 treated mitochondria with the calpain inhibitors, calpeptin or MDL28170 or with Ca(2+) chelator, EGTA does not cleave NCX. Treatment of the mitochondria with A23187 increases Ca(2+) level in the mitochondria, which subsequently dissociates mu-calpain-calpastatin association leading to the activation of mu-calpain. Immunoblot study of the A23187 treated mitochondria with the NCX polyclonal antibody indicates the degradation of mitochondrial inner membrane NCX (110kDa) resulting in the doublet of approximately 54-56kDa NCX fragments. Moreover, in vitro cleavage of mitochondrial purified NCX by mitochondrial purified mu-calpain supports our conclusion. This cleavage of NCX may be interpreted as the main cause of Ca(2+) overload and could lay a key role in the activation of apoptotic process in pulmonary smooth muscle.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-dihexadecyl-sn-glycero-3-phospho...,
http://linkedlifedata.com/resource/pubmed/chemical/1,2-oleoylphosphatidylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Calpain,
http://linkedlifedata.com/resource/pubmed/chemical/Dipeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipid Ethers,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Calcium Exchanger,
http://linkedlifedata.com/resource/pubmed/chemical/calpain inhibitor III,
http://linkedlifedata.com/resource/pubmed/chemical/calpastatin,
http://linkedlifedata.com/resource/pubmed/chemical/calpeptin,
http://linkedlifedata.com/resource/pubmed/chemical/mu-calpain
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1096-0384
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
482
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
66-76
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pubmed:meshHeading |
pubmed-meshheading:19094959-Animals,
pubmed-meshheading:19094959-Calcimycin,
pubmed-meshheading:19094959-Calcium,
pubmed-meshheading:19094959-Calcium-Binding Proteins,
pubmed-meshheading:19094959-Calpain,
pubmed-meshheading:19094959-Cattle,
pubmed-meshheading:19094959-Dipeptides,
pubmed-meshheading:19094959-Intracellular Membranes,
pubmed-meshheading:19094959-Mitochondria, Muscle,
pubmed-meshheading:19094959-Mitochondrial Membranes,
pubmed-meshheading:19094959-Muscle, Smooth, Vascular,
pubmed-meshheading:19094959-Phosphatidylcholines,
pubmed-meshheading:19094959-Phospholipid Ethers,
pubmed-meshheading:19094959-Pulmonary Artery,
pubmed-meshheading:19094959-Sodium-Calcium Exchanger
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pubmed:year |
2009
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pubmed:articleTitle |
mu-Calpain mediated cleavage of the Na+/Ca2+ exchanger in isolated mitochondria under A23187 induced Ca2+ stimulation.
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pubmed:affiliation |
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, India.
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pubmed:publicationType |
Journal Article
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