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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-10-9
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pubmed:abstractText |
Poly(dG-dC).poly(dG-dC) was modified by the reaction with 4-hydroxyaminoquinoline 1-oxide (4HAQO) in the presence of seryl-AMP. The conformations of 4HAQO-modified poly(dG-dC).poly(dG-dC) and of poly(dG-dC).poly(dG-dC) were studied by circular dichroism spectra under various salt concentration conditions. 4HAQO residues to guanine bases are inefficient in inducing the transition of poly(dG-dC).poly(dG-dC) from B-form to Z-form conformation. We have elicited monoclonal antibodies against 4HAQO-poly(dG-dC).poly(dG-dC). They were characterized using enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and binding to supercoiled DNA. These antibodies reacted with 4HAQO-poly(dG-dC).poly(dG-dC) specifically but not with 4HAQO-modified DNA or poly(dG).poly(dC). However, they cross-reacted with N-acetoxy-2-acetylaminofluorene-modified poly(dG-dC).poly(dG-dC) in Z-form conformation. These monoclonal antibodies may recognize a unique conformation in poly(dG-dC).poly(dG-dC) after 4HAQO modification.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-Hydroxyaminoquinoline-1-oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Acetoxyacetylaminofluorene,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Monophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Superhelical,
http://linkedlifedata.com/resource/pubmed/chemical/Poly C,
http://linkedlifedata.com/resource/pubmed/chemical/Poly G,
http://linkedlifedata.com/resource/pubmed/chemical/Polydeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Serine,
http://linkedlifedata.com/resource/pubmed/chemical/poly(dC),
http://linkedlifedata.com/resource/pubmed/chemical/poly(dC-dG),
http://linkedlifedata.com/resource/pubmed/chemical/poly(dG)
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
1090
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
29-37
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1909180-4-Hydroxyaminoquinoline-1-oxide,
pubmed-meshheading:1909180-Acetoxyacetylaminofluorene,
pubmed-meshheading:1909180-Adenosine Monophosphate,
pubmed-meshheading:1909180-Antibodies, Monoclonal,
pubmed-meshheading:1909180-Circular Dichroism,
pubmed-meshheading:1909180-DNA, Superhelical,
pubmed-meshheading:1909180-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1909180-Nucleic Acid Conformation,
pubmed-meshheading:1909180-Poly C,
pubmed-meshheading:1909180-Poly G,
pubmed-meshheading:1909180-Polydeoxyribonucleotides,
pubmed-meshheading:1909180-Radioimmunoassay,
pubmed-meshheading:1909180-Sensitivity and Specificity,
pubmed-meshheading:1909180-Serine,
pubmed-meshheading:1909180-Spectrophotometry, Ultraviolet
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pubmed:year |
1991
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pubmed:articleTitle |
Spectrophotometrical and immunochemical studies on the conformational changes in poly(dG-dC).poly(dG-dC) after modification by 4-hydroxyaminoquinoline 1-oxide.
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pubmed:affiliation |
Laboratory of Biochemistry, Aichi Cancer Center, Nagoya, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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