Linked Life Data

19088916

Source:http://linkedlifedata.com/resource/pubmed/id/19088916

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-12-17
pubmed:abstractText
Consecutively collected synovial fluids were examined for presence of bacterial DNA (a 700-bp fragment of the bacterial 23S rRNA gene) followed by DNA sequencing of amplicons, and by conventional bacteriological methods. One or more microorganisms were identified in 22 of the 227 synovial fluids (9,7%) originating from 17 patients. Sixteen of the patients had clinical signs of arthritis. For 11 patients molecular and conventional bacterial examinations were in agreement. Staphylococcus aureus, Streptococcus dysgalactiae subspecies equisimilis and Streptococcus pneumoniae, were detected in synovial fluids from 6, 2 and 2 patients, respectively. In 3 patients only 23S rRNA analysis was positive; 2 synovial fluids contained S. dysgalactiae subspecies equisimilis and 1 S. pneumoniae). The present study indicates a significant contribution by PCR with subsequent DNA sequencing of the 23S rRNA gene analysis in recognizing and identification of microorganisms from synovial fluids.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-10488193, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-10524694, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-10618059, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-11171695, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-12202592, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-12574279, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-14766807, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-16524406, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-17127195, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-17405973, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-17956825, http://linkedlifedata.com/resource/pubmed/commentcorrection/19088916-9254694
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:issn
1874-2858
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
85-8
pubmed:year
2008
pubmed:articleTitle
Infective Arthritis: Bacterial 23S rRNA Gene Sequencing as a Supplementary Diagnostic Method.
pubmed:affiliation
Department of Bacteriology, Mycology, and Parasitology, Statens Serum Institut, Copenhagen, and the Departments of Clinical Microbiology.
pubmed:publicationType
Journal Article