| pubmed-article:19088454 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19088454 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:19088454 | lifeskim:mentions | umls-concept:C1335204 | lld:lifeskim |
| pubmed-article:19088454 | lifeskim:mentions | umls-concept:C1418440 | lld:lifeskim |
| pubmed-article:19088454 | lifeskim:mentions | umls-concept:C2610604 | lld:lifeskim |
| pubmed-article:19088454 | pubmed:issue | 5-6 | lld:pubmed |
| pubmed-article:19088454 | pubmed:dateCreated | 2008-12-17 | lld:pubmed |
| pubmed-article:19088454 | pubmed:abstractText | Pharmacological inhibition of phosphoinositide 3-kinase (PI3K) has previously been shown to enhance basal gastric acid secretion. Signaling of PI-3-kinase includes activation of the phosphoinositide dependent kinase PDK1. We thus hypothesized that PDK1 may influence gastric acid secretion. In the present study gastric acid secretion in mice expressing ñ20 % of PDK1 (pdk1(hm)) was compared to gastric acid secretion in their wild type littermates (pdk1(wt)). According to BCECF-fluorescence cytosolic pH in isolated gastric glands was similar in pdk1(hm) and in pdk1(wt) mice. Na(+)-independent pH recovery (DeltapH/min) following an ammonium pulse, which reflects K(+)/H(+)-ATPase activity, was however, significantly faster in pdk1(hm) than in pdk1(wt) mice. In both genotypes, DeltapH/min was abolished in the presence of K(+)/H(+)-ATPase inhibitor omeprazole (100 microM). Increase of local K(+)-concentrations to 35 mM (replacing Na(+)/NMDG) significantly increased DeltapH/min in both, pdk1(hm) and pdk1(wt) mice, and abrogated the differences between genotypes. Similarly, treatment with 5 microM forskolin as well as stimulation of protein kinase C with phorbolester phorbol 12 myristate 13 acetate (100 nM) enhanced DeltapH/min to almost identical values in pdk1(hm) and in pdk1(wt)mice. Protein kinase A inhibitor H89 (50 nM) decreased DeltapH/min in pdk1(hm) to values similar to those in pdk1(wt). In conclusion, deficient activity of PDK1 leads to a marked increase of gastric acid secretion. The present observations thus disclose a novel element in the regulation of gastric H(+) secretion. | lld:pubmed |
| pubmed-article:19088454 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19088454 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19088454 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19088454 | pubmed:issn | 1421-9778 | lld:pubmed |
| pubmed-article:19088454 | pubmed:author | pubmed-author:LangFlorianF | lld:pubmed |
| pubmed-article:19088454 | pubmed:author | pubmed-author:BoiniKrishna... | lld:pubmed |
| pubmed-article:19088454 | pubmed:author | pubmed-author:RotteAnandA | lld:pubmed |
| pubmed-article:19088454 | pubmed:author | pubmed-author:BhandaruMadhu... | lld:pubmed |
| pubmed-article:19088454 | pubmed:author | pubmed-author:AckermannTere... | lld:pubmed |
| pubmed-article:19088454 | pubmed:copyrightInfo | Copyright 2008 S. Karger AG, Basel. | lld:pubmed |
| pubmed-article:19088454 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19088454 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:19088454 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19088454 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19088454 | pubmed:pagination | 725-34 | lld:pubmed |
| pubmed-article:19088454 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:meshHeading | pubmed-meshheading:19088454... | lld:pubmed |
| pubmed-article:19088454 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:19088454 | pubmed:articleTitle | Role of PDK1 in regulation of gastric acid secretion. | lld:pubmed |
| pubmed-article:19088454 | pubmed:affiliation | Department of Physiology, University of Tübingen, Tübingen, Germany. | lld:pubmed |
| pubmed-article:19088454 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19088454 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:18607 | entrezgene:pubmed | pubmed-article:19088454 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19088454 | lld:entrezgene |
