Linked Life Data

1908836

Source:http://linkedlifedata.com/resource/pubmed/id/1908836

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-10-1
pubmed:abstractText
The effect of mouse testicular extract (TE) on lymphocyte activation was investigated. TE, in the dose range 75-600 micrograms ml-1, suppressed significantly the blastogenic response of splenocytes to concanavalin A (Con-A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA) and lipopolysaccharide (LPS). TE also suppressed the blastogenic response of B-cells to LPS and of T-cells to PHA in a dose-dependent manner as well as suppressing the mixed lymphocyte reaction (MLR). Pretreatment of splenocytes with TE did not however, completely suppress their blastogenic response to Con-A, when the treated cells were washed prior to culturing. Furthermore, TE did not inhibit the on-going blastogenesis of splenocytes that had been activated already with Con-A for 48 h. Splenocytes obtained from TE-treated mice remained capable of responding to Con-A stimulation, whereas they did not respond to listerial antigens when mice were immunized with Listeria monocytogenes together with TE. The effects of TE were enhanced significantly by heating to 100 degrees C, but were resistant to pronase, RNase and DNase. These results suggest that TE affects non-specifically the stage of lymphocyte sensitization to antigens or mitogens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0105-6263
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
291-302
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Suppressive effect of a mouse testicular extract on lymphocyte activation.
pubmed:affiliation
Department of Bacteriology, Nara Medical University, Japan.
pubmed:publicationType
Journal Article