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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-9-27
|
| pubmed:abstractText |
A 67000 Mr bovine pregnancy-associated glycoprotein (bPAG) has been isolated from fetal cotyledons and purified to homogeneity by HPLC. The purification was monitored by a double immunodiffusion test and by RIA in conjunction with an antiserum raised against a crude fraction of placenta-specific antigens. The molecular weight of bPAG was estimated to be 67000 by SDS-PAGE. The isoelectric points (pI) of the four isoforms, determined by high-resolution analytical electrofocusing in polyacrylamide gel, were 4.4, 4.6, 5.2, and 5.4. The carbohydrate content of the bPAG consisted of approximately 10.02 +/- 1.09% neutral sugar and variant amounts of sialic acid (from 0.29 +/- 0.06% in the most basic isoform to 2.1 +/- 0.31% in the most acidic isoform). A specific antiserum was raised against the purified bPAG. A specific RIA showed that the bPAG was antigenically unrelated to BSA, alphafetoprotein (AFP), and human schwangerschafts-spezifischen (pregnancy-specific) beta 1 glycoprotein (SP1). According to some characteristics (e.g. the molecular weight), the purified bPAG may correspond to a form of the pregnancy-specific protein B previously described by Sasser and colleagues (Biol Reprod 1986; 35:936-942).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrates,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/N-Acetylneuraminic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Pregnancy-Specific beta...,
http://linkedlifedata.com/resource/pubmed/chemical/Sialic Acids
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0006-3363
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
45
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1-10
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1908709-Amino Acid Sequence,
pubmed-meshheading:1908709-Amino Acids,
pubmed-meshheading:1908709-Animals,
pubmed-meshheading:1908709-Carbohydrates,
pubmed-meshheading:1908709-Cattle,
pubmed-meshheading:1908709-Chromatography, High Pressure Liquid,
pubmed-meshheading:1908709-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1908709-Female,
pubmed-meshheading:1908709-Immune Sera,
pubmed-meshheading:1908709-Immunoblotting,
pubmed-meshheading:1908709-Immunodiffusion,
pubmed-meshheading:1908709-Isoelectric Focusing,
pubmed-meshheading:1908709-Molecular Sequence Data,
pubmed-meshheading:1908709-Molecular Weight,
pubmed-meshheading:1908709-N-Acetylneuraminic Acid,
pubmed-meshheading:1908709-Pregnancy,
pubmed-meshheading:1908709-Pregnancy-Specific beta 1-Glycoproteins,
pubmed-meshheading:1908709-Radioimmunoassay,
pubmed-meshheading:1908709-Sialic Acids
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Purification and characterization of a bovine pregnancy-associated glycoprotein.
|
| pubmed:affiliation |
Département d'Endocrinologie et de Reproduction Animales (Unité de Recherche IRSIA) Faculté de Médecine Vétérinaire, Bruxelles, Belgique.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|