Linked Life Data

19084994

Source:http://linkedlifedata.com/resource/pubmed/id/19084994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-2-24
pubmed:abstractText
In land plants plastocyanin is indispensable and therefore copper (Cu) availability is a prerequisite for growth. When Cu supply is limited, higher plants prioritize the Cu delivery to plastocyanin by down-regulation of other Cu proteins. Arabidopsis has two plastocyanin genes (PETE1 and PETE2). PETE2 is the predominant isoform in soil-grown plants and in hydroponic cultures it is accumulated in response to Cu addition. It functions as a Cu sink when more Cu is available, in addition to its role as an electron carrier. PETE1 is not affected by Cu feeding and it is the isoform that drives electron transport under Cu-deficiency. Cu feeding rescued the defect in photosystem II electron flux (Phi(PSII)) in the pete1 mutant whereas Phi(PSII) was not changed in the pete2 mutant as Cu was added. Plants with mutations in the plastocyanin genes had altered Cu homeostasis. The pete2 mutant accumulated more Cu/Zn superoxide dismutase (CSD2 and CSD1) and Cu chaperone (CCS) whereas the pete1 mutant accumulated less. On the other hand, less iron superoxide dismutase (FeSOD) and microRNA398b were observed in the pete2 mutant, whereas more were accumulated in the pete1 mutant. Our data suggest that plastocyanin isoforms are different in their response to Cu and the absence of either one changes the Cu homeostasis. Also a small amount of plastocyanin is enough to support efficient electron transport and more PETE2 is accumulated as more Cu is added, presumably, to buffer the excess Cu.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1432-2048
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
229
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
767-79
pubmed:meshHeading
pubmed-meshheading:19084994-Arabidopsis, pubmed-meshheading:19084994-Arabidopsis Proteins, pubmed-meshheading:19084994-Copper, pubmed-meshheading:19084994-Cytochrome b6f Complex, pubmed-meshheading:19084994-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:19084994-Gene Expression Profiling, pubmed-meshheading:19084994-Gene Expression Regulation, Developmental, pubmed-meshheading:19084994-Gene Expression Regulation, Plant, pubmed-meshheading:19084994-Homeostasis, pubmed-meshheading:19084994-Immunoblotting, pubmed-meshheading:19084994-Mutation, pubmed-meshheading:19084994-Photosynthesis, pubmed-meshheading:19084994-Photosystem I Protein Complex, pubmed-meshheading:19084994-Photosystem II Protein Complex, pubmed-meshheading:19084994-Plastocyanin, pubmed-meshheading:19084994-Protein Isoforms, pubmed-meshheading:19084994-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19084994-Ribulose-Bisphosphate Carboxylase, pubmed-meshheading:19084994-Superoxide Dismutase
pubmed:year
2009
pubmed:articleTitle
Contribution of plastocyanin isoforms to photosynthesis and copper homeostasis in Arabidopsis thaliana grown at different copper regimes.
pubmed:affiliation
Biology Department, Program in Molecular Plant Biology, Colorado State University, Fort Collins, CO 80523, USA. salahghany@gmail.com
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.