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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-9-25
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pubmed:abstractText |
Internalization of biotin-S-S-125I-transferrin (125I-BSST) into semiintact A431 cells were assessed by two different criteria which have allowed us to distinguish partial reactions in the complex overall process of receptor-mediated endocytosis. Early events resulting in the sequestration of ligand into deeply invaginated coated pits were measured by inaccessibility of 125I-BSST to exogenously added antibodies. Later events involving coated vesicle budding and membrane fission were measured by resistance of 125I-BSST to reduction by the membrane impermeant-reducing agent, MesNa. Acquisition of Ab inaccessibility occurred very efficiently in this cell-free system (approximately 50% of total cell-associated 125I-BSST became inaccessible) and could be inhibited by anti-clathrin mAbs and by antibodies directed against the cytoplasmic domain of the transferrin-receptor. In contrast, acquisition of MesNa resistance occurred less efficiently (approximately 10-20% of total cell-associated 125I-BSST) and showed differential sensitivity to inhibition by anti-clathrin and anti-transferrin receptor mAbs. Both partial reactions were stimulated by ATP and cytosol; indicating at least two ATP-requiring events in receptor-mediated endocytosis. The temperature dependence of both reactions was similar to that for 125I-BSST internalization in intact cells with no activity being observed below 10 degrees C. Morphological studies using gold-labeled ligands confirmed that internalization of transferrin receptors into semiintact A431 cell occurred via coated pits and coated vesicles and resulted in delivery of ligand to endosomal structures.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-1968060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-1973890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2126013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2163108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2177341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2209555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2257624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2298808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2407590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2415533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2557061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2564003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2565904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2574457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2576383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2857182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2864349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2883727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2900073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2904698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-2990347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-3009438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-3096714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-3111717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-3965443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-3968176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-6243633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-6309781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-6309862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908470-6831562
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
869-80
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1908470-Adenosine Triphosphate,
pubmed-meshheading:1908470-Antibodies, Monoclonal,
pubmed-meshheading:1908470-Cell-Free System,
pubmed-meshheading:1908470-Clathrin,
pubmed-meshheading:1908470-Coated Pits, Cell-Membrane,
pubmed-meshheading:1908470-Cytosol,
pubmed-meshheading:1908470-Endocytosis,
pubmed-meshheading:1908470-Humans,
pubmed-meshheading:1908470-Immunologic Techniques,
pubmed-meshheading:1908470-Microscopy, Electron,
pubmed-meshheading:1908470-Receptors, Transferrin,
pubmed-meshheading:1908470-Temperature,
pubmed-meshheading:1908470-Transferrin,
pubmed-meshheading:1908470-Tumor Cells, Cultured
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pubmed:year |
1991
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pubmed:articleTitle |
Stage-specific assays for coated pit formation and coated vesicle budding in vitro.
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pubmed:affiliation |
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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