Source:http://linkedlifedata.com/resource/pubmed/id/19084593
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2009-1-26
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pubmed:abstractText |
TWINKLE is a DNA helicase needed for mitochondrial DNA replication. In lower eukaryotes the protein also harbors a primase activity, which is lost from TWINKLE encoded by mammalian cells. Mutations in TWINKLE underlie autosomal dominant progressive external ophthalmoplegia (adPEO), a disorder associated with multiple deletions in the mtDNA. Four different adPEO-causing mutations (W315L, K319T, R334Q, and P335L) are located in the N-terminal domain of TWINKLE. The mutations cause a dramatic decrease in ATPase activity, which is partially overcome in the presence of single-stranded DNA. The mutated proteins have defects in DNA helicase activity and cannot support normal levels of DNA replication. To explain the phenotypes, we use a molecular model of TWINKLE based on sequence similarities with the phage T7 gene 4 protein. The four adPEO-causing mutations are located in a region required to bind single-stranded DNA. These mutations may therefore impair an essential element of the catalytic cycle in hexameric helicases, i.e. the interplay between single-stranded DNA binding and ATP hydrolysis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
1792
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
132-9
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pubmed:meshHeading |
pubmed-meshheading:19084593-Amino Acid Sequence,
pubmed-meshheading:19084593-DNA, Mitochondrial,
pubmed-meshheading:19084593-DNA Helicases,
pubmed-meshheading:19084593-DNA Replication,
pubmed-meshheading:19084593-Models, Molecular,
pubmed-meshheading:19084593-Molecular Sequence Data,
pubmed-meshheading:19084593-Mutation,
pubmed-meshheading:19084593-Ophthalmoplegia, Chronic Progressive External,
pubmed-meshheading:19084593-Protein Structure, Quaternary,
pubmed-meshheading:19084593-Recombinant Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
Structure-function defects of the twinkle amino-terminal region in progressive external ophthalmoplegia.
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pubmed:affiliation |
Department of Laboratory Medicine, Division of Metabolic Diseases, Karolinska Institutet, Novum, SE-141 86 Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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