Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1991-9-18
pubmed:abstractText
The alpha i2 subunit of the inhibitory heterotrimeric guanine nucleotide-binding proteins is highly conserved in mammals and is expressed in all cell types, but its exact function is not yet defined. We have investigated the role of this protein by producing embryonic stem (ES) cells lacking a functional alpha i2 gene. These alpha i2-null cell lines regulate adenylyl cyclase and grow and differentiate in vitro the same as wild-type ES cells. Homologous recombination was used to sequentially inactivate both copies of the alpha i2 gene. The first allele was inactivated by insertion of a neomycin-resistance gene. We modified the hygromycin B-resistance gene for improved expression in ES cells and used this gene to inactivate the remaining normal allele. The techniques used should be generally applicable to other genes whether or not they are expressed in ES cells.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-1975492, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2116039, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2116665, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-222124, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2250720, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2511433, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2554496, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2563901, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2563902, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2645293, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2685607, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2820999, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2822260, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-2833889, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3033510, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3040750, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3092218, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3113327, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3130578, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3132707, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3139030, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3143915, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3143918, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3194019, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3440520, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3465038, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3683574, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-3897439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1908087-6151992
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7036-40
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:1908087-Adenylate Cyclase, pubmed-meshheading:1908087-Alleles, pubmed-meshheading:1908087-Animals, pubmed-meshheading:1908087-Base Sequence, pubmed-meshheading:1908087-Blotting, Northern, pubmed-meshheading:1908087-Blotting, Southern, pubmed-meshheading:1908087-Cell Differentiation, pubmed-meshheading:1908087-Cell Line, pubmed-meshheading:1908087-DNA, pubmed-meshheading:1908087-Embryo, Mammalian, pubmed-meshheading:1908087-GTP-Binding Proteins, pubmed-meshheading:1908087-Genomic Library, pubmed-meshheading:1908087-Macromolecular Substances, pubmed-meshheading:1908087-Mice, pubmed-meshheading:1908087-Molecular Sequence Data, pubmed-meshheading:1908087-Oligonucleotide Probes, pubmed-meshheading:1908087-Polymerase Chain Reaction, pubmed-meshheading:1908087-Restriction Mapping, pubmed-meshheading:1908087-Stem Cells, pubmed-meshheading:1908087-Transfection
pubmed:year
1991
pubmed:articleTitle
Embryonic stem cells lacking a functional inhibitory G-protein subunit (alpha i2) produced by gene targeting of both alleles.
pubmed:affiliation
Howard Hughes Medical Institute, Boston, MA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't