Source:http://linkedlifedata.com/resource/pubmed/id/19079233
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
2008-12-16
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pubmed:abstractText |
In ulcerative colitis, the T helper type 2 proinflammatory cytokine Interleukin-13 (IL-13) contributes as effector cytokine to the epithelial changes associated with disturbed epithelial barrier function. This study aimed to investigate the underlying mechanisms in a colonic epithelial cell culture model. For studying these epithelial features in response to proinflammatory cytokines epithelial apoptosis was investigated by TdT-mediated X-dUTP nick end labeling (TUNEL) staining in HT-29/B6 cell monolayers. In contrast to interferon-gamma, IL-13 significantly upregulated the apoptotic rate of cells, which was intensified by simultaneous exposure to tumor necrosis factor-alpha. That this has a direct functional influence on epithelial barrier was shown by the caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp, which inhibited IL-13 induced apoptosis induction and concomitantly reversed the decrease in epithelial resistance by approximately 50%. Direct evidence for apoptotic rosettes at corresponding sites of barrier defects in the epithelium was obtained by conductance scanning. In addition, the pore-forming tight junction protein claudin-2 was found to be upregulated at protein and mRNA level. In conclusion, IL-13 disturbs intestinal barrier function through mechanisms including apoptosis induction and alteration of tight junction protein composition.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CLDN2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1935-3456
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
1 Suppl 1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
S58-61
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pubmed:meshHeading |
pubmed-meshheading:19079233-Animals,
pubmed-meshheading:19079233-Apoptosis,
pubmed-meshheading:19079233-Cell Line,
pubmed-meshheading:19079233-Cytokines,
pubmed-meshheading:19079233-Epithelial Cells,
pubmed-meshheading:19079233-Epithelium,
pubmed-meshheading:19079233-Humans,
pubmed-meshheading:19079233-Inflammation,
pubmed-meshheading:19079233-Inflammation Mediators,
pubmed-meshheading:19079233-Inflammatory Bowel Diseases,
pubmed-meshheading:19079233-Interleukin-13,
pubmed-meshheading:19079233-Membrane Proteins,
pubmed-meshheading:19079233-Tight Junctions
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pubmed:year |
2008
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pubmed:articleTitle |
Epithelial apoptosis is a prominent feature of the epithelial barrier disturbance in intestinal inflammation: effect of pro-inflammatory interleukin-13 on epithelial cell function.
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pubmed:affiliation |
Department of Gastroenterology, Infectious Diseases and Rheumatology, Charité, Campus Benjamin Franklin, Berlin, Germany.
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pubmed:publicationType |
Journal Article
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