19076284

Source:http://linkedlifedata.com/resource/pubmed/id/19076284

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0003737, umls-concept:C0006837, umls-concept:C0081786, umls-concept:C1171318, umls-concept:C1280412
pubmed:issue	5
pubmed:dateCreated	2009-8-19
pubmed:abstractText	Biofilm formation plays an integral role in catheter-associated bloodstream infections caused by Candida albicans. Biofilms formed on catheters placed intravenously are exposed to shear stress caused by blood flow. In this study, we investigated whether shear stress affects the ability of C. albicans to form biofilms. Candida biofilms were formed on catheter discs and exposed to physiological levels of shear stress using a rotating disc system (RDS). Control biofilms were grown under conditions of no flow. Tetrazolium (XTT) assay and dry weight (DW) measurements were used to quantify metabolic activity and biofilm mass respectively. Confocal scanning laser microscopy (CSLM) was used to evaluate architecture and biofilm thickness. After 90 min, cells attached under no-flow exhibited significantly greater XTT activity and DW than those under shear. However, by 24 h, biofilms formed under both conditions had similar XTT activities and DW. Interestingly, thickness of biofilms formed under no-flow was significantly greater after 24 h than of those formed under shear stress, demonstrating that shear exposure results in thinner, but denser biofilms. These studies suggest that biofilm architecture is modulated by shear in a phase-dependent manner.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/1R01 DE017846, http://linkedlifedata.com/resource/pubmed/grant/P30 AR039750-17, http://linkedlifedata.com/resource/pubmed/grant/P30 AR039750-18, http://linkedlifedata.com/resource/pubmed/grant/P30-AR-39750, http://linkedlifedata.com/resource/pubmed/grant/P30CA43703, http://linkedlifedata.com/resource/pubmed/grant/R01 EB00279
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pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/8805008
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/1,5-bis(2-methoxy-4-nitro-5-sulfophe..., http://linkedlifedata.com/resource/pubmed/chemical/Formazans
pubmed:status	MEDLINE
pubmed:month	Sep
pubmed:issn	1439-0507
pubmed:author	pubmed-author:AndersonJames MJM, pubmed-author:ChandDavid VDV, pubmed-author:ChandraJyotsnaJ, pubmed-author:GhannoumMahmoud AMA, pubmed-author:MukherjeePranab KPK
pubmed:issnType	Electronic
pubmed:volume	52
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	440-6
pubmed:dateRevised	2011-8-1
pubmed:meshHeading	pubmed-meshheading:19076284-Biofilms, pubmed-meshheading:19076284-Biomass, pubmed-meshheading:19076284-Candida albicans, pubmed-meshheading:19076284-Catheterization, pubmed-meshheading:19076284-Equipment and Supplies, pubmed-meshheading:19076284-Formazans, pubmed-meshheading:19076284-Humans, pubmed-meshheading:19076284-Microbial Viability, pubmed-meshheading:19076284-Microscopy, Confocal, pubmed-meshheading:19076284-Stress, Physiological
pubmed:year	2009
pubmed:articleTitle	Shear stress modulates the thickness and architecture of Candida albicans biofilms in a phase-dependent manner.
pubmed:affiliation	Center for Medical Mycology, Department of Dermatology, University Hospitals of Cleveland and Case Western Reserve University, OH 44106-5028, USA.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural