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pubmed-article:1907511pubmed:abstractTextThe nucleotide sequence and derived amino acid sequence of two different beta-glucosidase cDNA clones were determined. One clone (TRE104) was identified as the cyanogenic beta-glucosidase by homology with the N-terminal and internal peptide amino acid sequence of the purified enzyme. The biological function of the other beta-glycosidase (TRE361) is not known. Co-segregation of genomic restriction fragments uniquely identified by each cDNA clone shows that these two genes are linked in the white clover genome. Both TRE104 and TRE361 fragments co-segregate with cyanogenic beta-glucosidase activity. Extensive homology was found between the white clover beta-glucosidase sequences and a group of prokaryote and mammalian beta-glycosidases. This group of sequences has no homology with a separate set of beta-glucosidase genes isolated from fungi and the thermophilic bacterium Clostridium thermocellum.lld:pubmed
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pubmed-article:1907511pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1907511pubmed:articleTitleNucleotide and derived amino acid sequence of the cyanogenic beta-glucosidase (linamarase) from white clover (Trifolium repens L.).lld:pubmed
pubmed-article:1907511pubmed:affiliationDepartment of Biochemistry and Genetics, University of Newcastle upon Tyne, UK.lld:pubmed
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