|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1991-9-12
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The nucleotide sequence and derived amino acid sequence of two different beta-glucosidase cDNA clones were determined. One clone (TRE104) was identified as the cyanogenic beta-glucosidase by homology with the N-terminal and internal peptide amino acid sequence of the purified enzyme. The biological function of the other beta-glycosidase (TRE361) is not known. Co-segregation of genomic restriction fragments uniquely identified by each cDNA clone shows that these two genes are linked in the white clover genome. Both TRE104 and TRE361 fragments co-segregate with cyanogenic beta-glucosidase activity. Extensive homology was found between the white clover beta-glucosidase sequences and a group of prokaryote and mammalian beta-glycosidases. This group of sequences has no homology with a separate set of beta-glucosidase genes isolated from fungi and the thermophilic bacterium Clostridium thermocellum.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0167-4412
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
17
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
209-19
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1907511-Amino Acid Sequence,
pubmed-meshheading:1907511-Base Sequence,
pubmed-meshheading:1907511-Blotting, Northern,
pubmed-meshheading:1907511-Blotting, Southern,
pubmed-meshheading:1907511-Cloning, Molecular,
pubmed-meshheading:1907511-DNA,
pubmed-meshheading:1907511-Gene Expression,
pubmed-meshheading:1907511-Molecular Sequence Data,
pubmed-meshheading:1907511-Plants,
pubmed-meshheading:1907511-RNA, Messenger,
pubmed-meshheading:1907511-beta-Glucosidase
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Nucleotide and derived amino acid sequence of the cyanogenic beta-glucosidase (linamarase) from white clover (Trifolium repens L.).
|
|
pubmed:affiliation |
Department of Biochemistry and Genetics, University of Newcastle upon Tyne, UK.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
