Linked Life Data

19073710

Source:http://linkedlifedata.com/resource/pubmed/id/19073710

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-2-3
pubmed:abstractText
Preterm premature rupture of the membranes (PPROM) has been considered to be closely associated with chorioamnionitis. However, the detailed mechanism is not well understood. Alpha 1 antitrypsin (AAT) was reported to decrease in concentration in amniotic fluid obtained from patients with PPROM. However, the origin of AAT in amniotic fluid has not been clarified. In this study, we assessed the expression and localization of AAT in human amnion, as well as its biological activity in cases with PROM. Human amniotic epithelial (hAE) cells expressed AAT. After stimulation with oncostatin M (OSM), interleukin-6 (IL-6) or tumor necrotic factor alpha (TNF alpha), hAE cells increased the expression of AAT, while the expression of MMP9 was reduced by OSM and induced by TNF alpha. Oxidized AAT (inactivated form) was detected in the amnion with PPROM and TPROM, but not in specimens without PROM. Moreover, AAT activity was decreased in amnions from cases with PROM, regardless of gestational age. Thus, the results showed that AAT in the amnion may function as a protective shield at inflammatory sites, and not as it loses it inhibitory activity in cases with PROM, possibly by oxidation, suggesting that its imbalance contributes to PROM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1460-2407
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-57
pubmed:meshHeading
pubmed-meshheading:19073710-Adult, pubmed-meshheading:19073710-Amnion, pubmed-meshheading:19073710-Blotting, Western, pubmed-meshheading:19073710-Cells, Cultured, pubmed-meshheading:19073710-Female, pubmed-meshheading:19073710-Fetal Membranes, Premature Rupture, pubmed-meshheading:19073710-Gene Expression Regulation, pubmed-meshheading:19073710-Growth Inhibitors, pubmed-meshheading:19073710-Humans, pubmed-meshheading:19073710-Immunohistochemistry, pubmed-meshheading:19073710-Interleukin-6, pubmed-meshheading:19073710-Matrix Metalloproteinase 9, pubmed-meshheading:19073710-Oncostatin M, pubmed-meshheading:19073710-Pregnancy, pubmed-meshheading:19073710-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19073710-Tumor Necrosis Factor-alpha, pubmed-meshheading:19073710-alpha 1-Antitrypsin
pubmed:year
2009
pubmed:articleTitle
Alpha 1 antitrypsin activity is decreased in human amnion in premature rupture of the fetal membranes.
pubmed:affiliation
Department of Regenerative Medicine, University of Toyama Graduate School of Medicine and Pharmaceutical Sciences, Toyama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't