Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2008-12-16
pubmed:abstractText
In the present study, three Y-box cDNAs, named as Lyb1, Lyb2, and Lyb3, were cloned from the liver and oral gland of Japanese lamprey (Lampetra japonica) using reverse transcript-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques. Further bioinformatical analysis indicated that the three Y-box genes of lamprey, i.e., Lyb1, Lyb2, and Lyb3, encoded 331, 181, and 171 amino acids, respectively. The three amino acid sequences were homologically aligned with those from Ciona intestinalis, Danio rerio, Oryzias latipes, Takifugu rubripes, and Mus musculus by DNAMAN software. The Lyb genes were novel members of Y-box gene family containing a conserved cold-shock domain with two RNA binding motifs (RNP-1 and RNP-2). Moreover, phylogenetic analysis revealed that there was only one type of Y-box gene before the emergence of gnathostomata. By contrast, the Y-box gene were divided into three groups, YB1, YB2, and CSDA during evolution of jawed vertebrates.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0253-9772
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1597-602
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
[Cloning and analysis of Lyb gene from Lampetra japonica].
pubmed:affiliation
Institute of Functional Gene & Proteomics of Marine Biology Liaoning Normal University, Dalian 116029, China. jinping8312@163.com
pubmed:publicationType
Journal Article, English Abstract