Linked Life Data

19066017

Source:http://linkedlifedata.com/resource/pubmed/id/19066017

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-12-10
pubmed:abstractText
The transport of membrane-bound proteins through post-Golgi compartments depends on the coordinated function of multiple genes that direct the recognition and routing of protein cargoes to their final cellular destination. As many of these sorting components are nonessential for viability, genome-wide screening of the yeast gene-deletion mutant collection provides a useful strategy for their identification. The potential of this approach is limited only by the availability of transport assays suitable for the high-throughput screening of yeast colony arrays. Two large-scale phenotypic screens to identify novel transport genes are described here. The fluorescence-based Calcofluor white assay identifies mutants with altered plasma membrane localization of the chitin synthase Chs3, which recycles between the cell surface, endosomes, and the late Golgi. The carboxypeptidase Y (CPY) assay allows mutants of a distinct Golgi-to-vacuole transport pathway to be identified, due to the missorting and secretion of the vacuolar hydrolase CPY from the cell.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
457
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-39
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Genome-wide analysis of membrane transport using yeast knockout arrays.
pubmed:affiliation
Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, Bristish Columbia, Canada.
pubmed:publicationType
Journal Article