Linked Life Data

19066016

Source:http://linkedlifedata.com/resource/pubmed/id/19066016

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-12-10
pubmed:abstractText
A variety of Saccharomyces cerevisiae strain libraries allow for systematic analysis of strains bearing gene deletions, repressible genes, overexpressed genes, or modified genes on a genome-wide scale. Here we introduce a method for culturing yeast strains in 96-well format to achieve log-phase growth and a high-throughput technique for generating whole-cell protein extracts from these cultures using sodium dodecyl sulfate and heat lysis. We subsequently describe a procedure to analyze these whole-cell extracts by immunoblotting for alkaline phosphatase and carboxypeptidase yscS to identify strains with defects in protein transport pathways or protein glycosylation. These methods should be readily adaptable to many different areas of interest.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
457
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-27
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
High-throughput protein extraction and immunoblotting analysis in Saccharomyces cerevisiae.
pubmed:affiliation
Department of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural