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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
25
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pubmed:dateCreated |
2008-12-9
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pubmed:abstractText |
We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1094-4087
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pubmed:author |
pubmed-author:BossiMarianoM,
pubmed-author:EggelingChristianC,
pubmed-author:EgnerAlexanderA,
pubmed-author:GeislerClaudiaC,
pubmed-author:HellStefan WSW,
pubmed-author:JakobsStefanS,
pubmed-author:MeddaRebeccaR,
pubmed-author:SchönleAndreasA,
pubmed-author:StielAndre CAC,
pubmed-author:TestaIlariaI,
pubmed-author:WurmChristian ACA,
pubmed-author:von MiddendorffClaasC
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pubmed:issnType |
Electronic
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pubmed:day |
8
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pubmed:volume |
16
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
21093-104
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Nanoscale separation of molecular species based on their rotational mobility.
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pubmed:affiliation |
Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
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pubmed:publicationType |
Journal Article
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