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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
1991-8-19
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pubmed:abstractText |
Apolipoprotein A-I (apoA-I) participates in transport of plasma cholesterol. Concentrations of apoA-I depend on the balance between production and fractional clearance. To elucidate factors influencing apoA-I levels, accurate estimates of apoA-I turnover rates may be valuable. We describe a method for isolation of autologous apoA-I and its use in turnover studies. Free apoA-I was isolated from high density lipoproteins (HDL) by treatment with guanidine hydrochloride. This free apoA-I was radioiodinated with 131I and injected into eleven subjects simultaneously with HDL labeled with 125I. Plasma die-away curves of free apoA-I (131I) and HDL apoA-I (125I) were compared; fractional clearance rates averaged 0.256 +/- 0.019 (SEM) and 0.254 +/- 0.017 pools/day, respectively. Although slight differences between the two die-away curves were noted for some of the patients, the differences were relatively small; for the group as a whole, average fractional catabolic rates were not significantly different. Thus, by isolation of autologous apoA-I under the conditions described, free apoA-I seemingly provides a valid method for estimating apoA-I turnover.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-2275
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
32
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
867-75
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1906523-Adult,
pubmed-meshheading:1906523-Aged,
pubmed-meshheading:1906523-Apolipoprotein A-I,
pubmed-meshheading:1906523-Apolipoproteins A,
pubmed-meshheading:1906523-Evaluation Studies as Topic,
pubmed-meshheading:1906523-Humans,
pubmed-meshheading:1906523-Kinetics,
pubmed-meshheading:1906523-Lipoproteins, HDL,
pubmed-meshheading:1906523-Male,
pubmed-meshheading:1906523-Methods,
pubmed-meshheading:1906523-Middle Aged
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pubmed:year |
1991
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pubmed:articleTitle |
Evaluation of a method for study of kinetics of autologous apolipoprotein A-I.
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pubmed:affiliation |
Department of Clinical Nutrition, University of Texas Southwestern Medical Center, Dallas.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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