Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-1-23
pubmed:abstractText
Complete transcriptome profiling of contractile smooth muscle cells (SMCs) differentiated from embryonic stem cells is crucial for the characterization of smooth muscle gene expression signatures and will contribute to defining biological and physiological processes in these cells. We have generated a transgenic embryonic stem cell line expressing both the puromycin acetyl transferase and enhanced green fluorescent protein cassettes under the control of the Acta2 promoter. Applying a specific monolayer culture protocol using retinoic acid, a puromycin-resistant and enhanced green fluorescent protein-positive Acta2(+) SMC population of 95% purity was isolated. Acta2(+) SMCs were characterized by semiquantitative and quantitative RT-PCR profiling of SMC markers and by microarray expression profiling, as well as by immunostaining for SMC-specific cytoskeletal proteins. Patch-clamp electrophysiological characterization of these cells identified SMC-specific channels such as the ATP-sensitive potassium channel and the Ca(2+)-activated potassium channel. Culturing of Acta2(+) SMCs in serum-containing medium resulted in a significant number of hypertrophic and binucleated cells failing to complete cell division. Functional characterization of the cells has been proved by stimulation of the cells with vasoactive agents, such as angiotensin II and endothelin. We concluded that our embryonic stem cell-derived SMC population possesses the contractile and hypertrophic phenotype of SMCs incapable of proliferation. This is the first study describing the complete transcriptome of ES-derived SMCs allowing identification of specific biological and physiological processes in the contractile phenotype SMCs and will contribute to the understanding of these processes in early SMCs derived from embryonic stem cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Actins, http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Desmin, http://linkedlifedata.com/resource/pubmed/chemical/Endothelins, http://linkedlifedata.com/resource/pubmed/chemical/KATP Channels, http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Myosin Heavy Chains, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels..., http://linkedlifedata.com/resource/pubmed/chemical/calponin, http://linkedlifedata.com/resource/pubmed/chemical/transgelin
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1524-4563
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
196-204
pubmed:dateRevised
2011-11-10
pubmed:meshHeading
pubmed-meshheading:19064816-Actins, pubmed-meshheading:19064816-Angiotensin II, pubmed-meshheading:19064816-Animals, pubmed-meshheading:19064816-Calcium-Binding Proteins, pubmed-meshheading:19064816-Cell Differentiation, pubmed-meshheading:19064816-Cells, Cultured, pubmed-meshheading:19064816-Desmin, pubmed-meshheading:19064816-Embryonic Stem Cells, pubmed-meshheading:19064816-Endothelins, pubmed-meshheading:19064816-Gene Expression Profiling, pubmed-meshheading:19064816-Hypertrophy, pubmed-meshheading:19064816-KATP Channels, pubmed-meshheading:19064816-Mice, pubmed-meshheading:19064816-Mice, Transgenic, pubmed-meshheading:19064816-Microfilament Proteins, pubmed-meshheading:19064816-Muscle Contraction, pubmed-meshheading:19064816-Muscle Proteins, pubmed-meshheading:19064816-Myocytes, Smooth Muscle, pubmed-meshheading:19064816-Myosin Heavy Chains, pubmed-meshheading:19064816-Patch-Clamp Techniques, pubmed-meshheading:19064816-Potassium Channels, Calcium-Activated
pubmed:year
2009
pubmed:articleTitle
Functional characterization and transcriptome analysis of embryonic stem cell-derived contractile smooth muscle cells.
pubmed:affiliation
University of Cologne, Center of Physiology and Pathophysiology, Institute of Neurophysiology, Robert Koch Str 39, Cologne, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't