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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2008-12-9
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pubmed:abstractText |
Alphaviruses infect cells via a low-pH-triggered membrane fusion reaction mediated by the class II virus fusion protein E1, an elongated molecule with three extramembrane domains (DI-III). E1 drives fusion by inserting its fusion peptide loop into the target membrane and refolding to a hairpin-like trimer in which DIII moves toward the target membrane and packs against the central trimer. Three-dimensional structures provide static pictures of prefusion and postfusion E1 but do not explain this transition. Using truncated forms of E1, we reconstituted a low-pH-dependent intermediate composed of trimers of DI/II. Unexpectedly, DI/II trimers were stable in the absence of DIII. Once formed at a low pH, DI/II trimers efficiently and specifically bound recombinant DIII through a pH-independent reaction. Even in the absence of DIII, DI/II trimers interacted to form hexagonal lattices and to cause membrane deformation and tubulation. These studies identify a prefusion intermediate in class II membrane fusion.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-10559317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-10644331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-10933683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-11301009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-11884551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-12759475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-13678581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-1370493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-1433520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-14737159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-14737160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-14963486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-15016852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-15341726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-15613349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-16216925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-16357862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-16364734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-16407066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-16407067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-16943291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-16987985,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-2118964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-7753193,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-8769412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19064260-9375004
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1934-6069
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
11
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
600-8
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pubmed:dateRevised |
2010-9-22
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pubmed:meshHeading |
pubmed-meshheading:19064260-Alphavirus,
pubmed-meshheading:19064260-Hydrogen-Ion Concentration,
pubmed-meshheading:19064260-Macromolecular Substances,
pubmed-meshheading:19064260-Microscopy, Electron, Transmission,
pubmed-meshheading:19064260-Models, Molecular,
pubmed-meshheading:19064260-Protein Binding,
pubmed-meshheading:19064260-Viral Fusion Proteins,
pubmed-meshheading:19064260-Virus Internalization
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pubmed:year |
2008
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pubmed:articleTitle |
A stable prefusion intermediate of the alphavirus fusion protein reveals critical features of class II membrane fusion.
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pubmed:affiliation |
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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