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pubmed-article:19061518pubmed:abstractTextIn bacteriology, the ability to grow in selective media and to form colonies on nutrient agar plates is routinely used as a retrospective criterion for the detection of living bacteria. However, the utilization of indicators for bacterial viability-such as the presence of specific transcripts or membrane integrity-would overcome bias introduced by cultivation and reduces the time span of analysis from initiation to read out. Therefore, we investigated the correlation between transcriptional activity, membrane integrity and cultivation-based viability in the Gram-positive model bacterium Bacillus subtilis.lld:pubmed
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pubmed-article:19061518pubmed:articleTitleTranscriptional activity around bacterial cell death reveals molecular biomarkers for cell viability.lld:pubmed
pubmed-article:19061518pubmed:affiliationTNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group, Utrechtseweg 48, Zeist, The Netherlands. remco.kort@tno.nllld:pubmed
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