|
pubmed:abstractText |
In bacteriology, the ability to grow in selective media and to form colonies on nutrient agar plates is routinely used as a retrospective criterion for the detection of living bacteria. However, the utilization of indicators for bacterial viability-such as the presence of specific transcripts or membrane integrity-would overcome bias introduced by cultivation and reduces the time span of analysis from initiation to read out. Therefore, we investigated the correlation between transcriptional activity, membrane integrity and cultivation-based viability in the Gram-positive model bacterium Bacillus subtilis.
|
|
pubmed:affiliation |
TNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group, Utrechtseweg 48, Zeist, The Netherlands. remco.kort@tno.nl
|
