Source:http://linkedlifedata.com/resource/pubmed/id/19059477
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2009-2-9
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| pubmed:abstractText |
Repetitive stress has been shown to up-regulate antioxidant defense and increase survival after subsequent oxidative injury. The up-regulation of antioxidant defense has been identified as an underlying cause of the apoptosis-inhibitory effects exerted by repetitive stress. However, it remains unclear what the important signaling mechanisms are by which cells preexposed to low-grade stress deal with apoptosis-inducing stress. In this study, we repetitively stressed human umbilical vein endothelial cells (HUVECs) through multiple exposures to a low dose (30 microM) of H(2)O(2) in culture for 4 weeks. We then examined the effects of repetitive stress on PPAR-beta expression and activity as well as the role of PPAR-beta in the protective potency of repetitive stress. Our results show that repetitive stress enhances PPAR-beta expression and activity, thereby inhibiting oxidative stress-induced apoptosis. Further, PPAR-beta-directed antisense oligonucleotides reduced the PPAR-beta protein content, enhanced the H(2)O(2)-mediated apoptosis, and ablated the protective effect of repetitive low-grade H(2)O(2) stress. The specific PPAR-beta agonist L-165041 significantly potentiated the apoptosis induced by H(2)O(2) (p<0.05) and increased the protective effect of repetitive stress. These findings indicate that repetitive low-grade H(2)O(2) stress protects HUVECs from subsequent oxidative stress-induced apoptosis by enhancing PPAR-beta expression and activity.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-(3-(2-propyl-3-hydroxy-4-acetyl)ph...,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/PPAR-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phenoxyacetates,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/SB 203580,
http://linkedlifedata.com/resource/pubmed/chemical/SN50 peptide,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1873-4596
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
1
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| pubmed:volume |
46
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
555-63
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| pubmed:meshHeading |
pubmed-meshheading:19059477-Apoptosis,
pubmed-meshheading:19059477-Cell Line,
pubmed-meshheading:19059477-Endothelial Cells,
pubmed-meshheading:19059477-Humans,
pubmed-meshheading:19059477-Hydrogen Peroxide,
pubmed-meshheading:19059477-Imidazoles,
pubmed-meshheading:19059477-NF-kappa B,
pubmed-meshheading:19059477-Oligonucleotides, Antisense,
pubmed-meshheading:19059477-Oxidative Stress,
pubmed-meshheading:19059477-PPAR-beta,
pubmed-meshheading:19059477-Peptides,
pubmed-meshheading:19059477-Phenoxyacetates,
pubmed-meshheading:19059477-Pyridines,
pubmed-meshheading:19059477-Signal Transduction,
pubmed-meshheading:19059477-Stress, Physiological,
pubmed-meshheading:19059477-Transcriptional Activation,
pubmed-meshheading:19059477-Umbilical Veins,
pubmed-meshheading:19059477-p38 Mitogen-Activated Protein Kinases
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| pubmed:year |
2009
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| pubmed:articleTitle |
Enhancement of PPAR-beta activity by repetitive low-grade H(2)O(2) stress protects human umbilical vein endothelial cells from subsequent oxidative stress-induced apoptosis.
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| pubmed:affiliation |
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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