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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2008-12-29
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pubmed:abstractText |
Previously, we demonstrated that protein kinase D (PKD) plays a protective role during H(2)O(2)-induced intestinal cell death. Here, we sought to determine whether this effect is mediated by nuclear factor-kappaB (NF-kappaB) and mitogen-activated protein kinases (MAPKs). Treatment with H(2)O(2) activated NF-kappaB in RIE-1 cells; H(2)O(2) also induced the translocation of NF-kappaB p65 as well as phosphorylation of IkappaB-alpha. PKD1 siRNA inhibited H(2)O(2)-induced activation, translocation of NF-kappaB, and phosphorylation of IkappaB-alpha. We also found that overexpression of wild type PKD1 attenuated H(2)O(2)-induced phosphorylation of p38 MAPK and its upstream activator, MAPK kinase (MKK) 3/6, whereas the phosphorylation was increased by PKD1 siRNA or kinase-dead PKD1. Phosphorylation of neither extracellular signal-regulated kinases (ERK) 1/2 nor c-Jun N-terminal kinases (JNK) was altered by PKD1 plasmids or siRNA. Our findings suggest that PKD protects intestinal cells through up-regulation of NF-kappaB and down-regulation of p38 MAPK.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-10197446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-10385605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-10646495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-10729185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-11242034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-11274345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-11371742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-11983155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-12115731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-12505989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-15024053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-15755722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-16166634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-16306192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-16421204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-17034759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-6204784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-8011280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-8078925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-9335256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-9430721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19059215-9865693
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1090-2104
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
16
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pubmed:volume |
378
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
610-4
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pubmed:dateRevised |
2011-4-22
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pubmed:meshHeading |
pubmed-meshheading:19059215-Animals,
pubmed-meshheading:19059215-Apoptosis,
pubmed-meshheading:19059215-Cell Line,
pubmed-meshheading:19059215-Enzyme Activation,
pubmed-meshheading:19059215-Hydrogen Peroxide,
pubmed-meshheading:19059215-I-kappa B Proteins,
pubmed-meshheading:19059215-Intestines,
pubmed-meshheading:19059215-MAP Kinase Kinase 3,
pubmed-meshheading:19059215-MAP Kinase Kinase 6,
pubmed-meshheading:19059215-Oxidative Stress,
pubmed-meshheading:19059215-Phosphorylation,
pubmed-meshheading:19059215-Protein Kinase C,
pubmed-meshheading:19059215-Protein Transport,
pubmed-meshheading:19059215-RNA, Small Interfering,
pubmed-meshheading:19059215-Rats,
pubmed-meshheading:19059215-Transcription Factor RelA,
pubmed-meshheading:19059215-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2009
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pubmed:articleTitle |
PKD prevents H2O2-induced apoptosis via NF-kappaB and p38 MAPK in RIE-1 cells.
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pubmed:affiliation |
Department of Surgery, The University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0353, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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