Linked Life Data

19054069

Source:http://linkedlifedata.com/resource/pubmed/id/19054069

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-1-7
pubmed:databankReference
pubmed:abstractText
AtlA is a major cell-lytic enzyme called autolysin in Streptococcus mutans. In this study, we identified the atlg gene-encoding autolysin (Atlg), consisting of 863 residues from Streptococcus sobrinus 6715DP, and confirmed lytic activity of recombinant Atlg by zymography of S. sobrinus cells. An atlA-inactivated mutant was constructed in S. mutans Xc, and the atlg gene product was characterized by plasmid complementation. Microscopic analysis, saliva-induced aggregation assay and autolysis assay of static cultures in air revealed that the atlg gene product partially complemented the role of AtlA. Furthermore, the capability of biofilm formation of the atlA-deficient mutant cultivated in air was restored by plasmid comprising the atlg gene. These findings suggest that Atlg may be involved in cell separation and biofilm formation in S. sobrinus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1574-6968
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
291
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17-23
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Identification and characterization of an autolysin gene, atlg, from Streptococcus sobrinus.
pubmed:affiliation
Department of Dental Pharmacology, School of Dentistry, Iwate Medical University, Morioka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't