Linked Life Data

19052961

Source:http://linkedlifedata.com/resource/pubmed/id/19052961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-12-4
pubmed:abstractText
Ion channels have been assigned a pivotal importance in various sperm functions and are therefore promising targets for contraceptive development. The lack of data on channel functionality and pharmacology has hampered this goal. This is a consequence of technical problems of applying electrophysiological techniques to spermatozoa due to their small size and form. By using a laminin coating to increase adherence of spermatozoa and nystatin in the patch pipette for pore formation, we have adapted the whole-cell recording technique to study currents in mature uncapacitated bovine spermatozoa. Employing these conditions, in the head region, patched spermatozoa could be transferred into the whole-cell configuration. For the first time we document an outward rectifying current in mature bovine spermatozoa was blocked by tetraethyl ammonium (TEA) chloride. The observation of a shift in the reversal potential as a response to changes in the extracellular concentration of K(+) ions allowed us to identify this current as K(+) selective. This result shows that K(+) channels in the head region of mature uncapacitated bovine spermatozoa can be suitably investigated using the whole-cell recording patch-clamp technique.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1939-6376
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
231-9
pubmed:dateRevised
2009-11-17
pubmed:meshHeading
pubmed:articleTitle
Potassium current in mature bovine spermatozoa.
pubmed:affiliation
Center for Reproductive Biotechnology, Laboratory of Neuroscience, Department of Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't