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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2008-12-3
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pubmed:abstractText |
All jawed vertebrates limit use of D(H) reading frames (RFs) that are enriched for hydrophobic amino acids. In BALB/c mice, DFL16.1 RF2 encodes valine and isoleucine. To test whether increased use of RF2 affects B cell function, we examined B cell development and Ab production in mice with an IgH allele (DeltaD-DmicroFS) limited to use of a single, frameshifted DFL61.1 gene segment. We compared the results of these studies to wild-type mice, as well as those previously obtained in mice limited to use of either a single normal D(H) or a single inverted D(H) that forces use of arginine in CDR-H3. All three of the mouse strains limited to a single D(H) produced fewer immature B cells than wild type. However, whereas mice limited to a single normal D(H) achieved normal B cell numbers in the periphery, mice forced to preferentially use RF2 had reduced numbers of mature B cells in the spleen and bone marrow, mirroring the pattern previously observed in mice enriched for charged CDR-H3s. There were two exceptions. B cells in the mice using RF2 normally populated the marginal zone and peritoneal cavity, whereas mice using inverted RF1 had increased numbers of marginal zone B cells and decreased numbers of B1a cells. When challenged with several T-dependent or T-independent Ags, Ag-specific Ab titers in the mice forced to use RF2 were altered. These findings indicate that B cell development and Ag-specific Ab production can be heavily influenced by the global amino acid content of the CDR-H3 repertoire.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/AI07051,
http://linkedlifedata.com/resource/pubmed/grant/AI42732,
http://linkedlifedata.com/resource/pubmed/grant/AI48115,
http://linkedlifedata.com/resource/pubmed/grant/F06 TW002130-01,
http://linkedlifedata.com/resource/pubmed/grant/HD043327,
http://linkedlifedata.com/resource/pubmed/grant/K08 HD043327-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI047322-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI048115-07,
http://linkedlifedata.com/resource/pubmed/grant/R21 AI088498-02,
http://linkedlifedata.com/resource/pubmed/grant/T32 AI007051-31A1,
http://linkedlifedata.com/resource/pubmed/grant/TW02130
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-10429672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-10837053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-10933393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-11244048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-11602729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-11739500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-12403364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-12574339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-15023420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-15367639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-15723805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-15944280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-16272317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-16501112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-16754718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-17174121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-17372000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-17475861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-17567700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-1786075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-2013094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-2583225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-4114394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-6095102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-6196436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-6205994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-8114766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-8568254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-8657279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-89036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-9245589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19050258-9341782
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1550-6606
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
181
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8409-15
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pubmed:dateRevised |
2011-5-11
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pubmed:meshHeading |
pubmed-meshheading:19050258-Animals,
pubmed-meshheading:19050258-Antibody Diversity,
pubmed-meshheading:19050258-B-Lymphocyte Subsets,
pubmed-meshheading:19050258-Cell Differentiation,
pubmed-meshheading:19050258-Complementarity Determining Regions,
pubmed-meshheading:19050258-Epitopes, B-Lymphocyte,
pubmed-meshheading:19050258-Female,
pubmed-meshheading:19050258-Frameshift Mutation,
pubmed-meshheading:19050258-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:19050258-Immunoglobulin Heavy Chains,
pubmed-meshheading:19050258-Mice,
pubmed-meshheading:19050258-Mice, Inbred BALB C,
pubmed-meshheading:19050258-Mice, Mutant Strains,
pubmed-meshheading:19050258-Reading Frames
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pubmed:year |
2008
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pubmed:articleTitle |
Preferential use of DH reading frame 2 alters B cell development and antigen-specific antibody production.
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pubmed:affiliation |
Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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