Linked Life Data

19048307

Source:http://linkedlifedata.com/resource/pubmed/id/19048307

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-3-11
pubmed:abstractText
The interaction between small molecules and telomeric quadruplex DNA has received great attention because of its importance in molecular recognition and anticancer drug design. Using UV/vis absorption titration, thermal melting, circular dichroism spectroscopy, and electrospray ionization mass spectrometry, we examined the formation of lead ion induced guanine quadruplexes (Pb-G4) from oligonucleotide AG(3)(T(2)AG(3))(3) and their interaction with a zinc derivative of 5,10,15,20-tetrakis(N-methyl-4-pyridyl)porphyrin (Zn-TMPyP). The binding of lead ion to the oligonucleotide was found to have an unusually high affinity and followed a 1:1 stoichiometry, and the resultant Pb-G4 structure was stabilized by Zn-TMPyP binding. Owing to the steric hindrance of the axial ligand of zinc and also the relatively rigid structure of Pb-G4, intercalation of Zn-TMPyP between adjacent guanine quartets is precluded, thus allowing the end-stacking binding mode to be characterized exclusively. In conjunction with a big redshift (more than 8 nm) in the absorption spectrum, we demonstrate that a conservative induced circular dichroism is an important signature for end-stacking of porphyrins on guanine quadruplexes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1432-1327
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Interaction between cationic zinc porphyrin and lead ion induced telomeric guanine quadruplexes: evidence for end-stacking.
pubmed:affiliation
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116012, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't