Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-1-15
pubmed:abstractText
Recent studies of 16S rRNA genes in the mammalian gut microbiota distinguished a higher Firmicutes/Bacteroidetes ratio in obese individuals compared to lean individuals. This ratio was estimated using a clonal Sanger sequencing approach which is time-consuming and requires laborious data analysis. In contrast, new high-throughput pyrosequencing technology offers an inexpensive alternative to clonal Sanger sequencing and would significantly advance our understanding of obesity via the development of a clinical diagnostic method. Here we present a cost-effective method that combines 16S rRNA pyrosequencing and DNA barcodes of the Firmicutes and Bacteroidetes 16S rRNA genes to determine the Firmicutes/Bacteroidetes ratio in the gut microbiota of obese humans.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-10068696, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-10964570, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-11472932, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-14985472, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15034147, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15333456, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15345463, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15661851, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15831718, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16033867, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16056220, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16820507, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16845028, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16845035, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16873492, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16880384, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17090583, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17183309, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17183312, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17468765, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17586664, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17686799, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17881377, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17916733, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18043639, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18047683, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18248093, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18285365, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18437230, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18665274, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18682840, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18818648, http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-9021275
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1471-2164
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
576
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Use of pyrosequencing and DNA barcodes to monitor variations in Firmicutes and Bacteroidetes communities in the gut microbiota of obese humans.
pubmed:affiliation
URMITE - UMR CNRS 6236, IRD 3R198, Université de la Méditerranée, Faculté de médecine, Marseille, France. fabrice.armougom@univmed.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't