rdf:type |
|
lifeskim:mentions |
umls-concept:C0004738,
umls-concept:C0009462,
umls-concept:C0012854,
umls-concept:C0028754,
umls-concept:C0086418,
umls-concept:C0181904,
umls-concept:C0205419,
umls-concept:C0596972,
umls-concept:C0699819,
umls-concept:C0728873,
umls-concept:C0995456,
umls-concept:C1000792,
umls-concept:C1254144,
umls-concept:C1521743,
umls-concept:C1524063,
umls-concept:C1704646,
umls-concept:C1956108,
umls-concept:C2737269
|
pubmed:dateCreated |
2009-1-15
|
pubmed:abstractText |
Recent studies of 16S rRNA genes in the mammalian gut microbiota distinguished a higher Firmicutes/Bacteroidetes ratio in obese individuals compared to lean individuals. This ratio was estimated using a clonal Sanger sequencing approach which is time-consuming and requires laborious data analysis. In contrast, new high-throughput pyrosequencing technology offers an inexpensive alternative to clonal Sanger sequencing and would significantly advance our understanding of obesity via the development of a clinical diagnostic method. Here we present a cost-effective method that combines 16S rRNA pyrosequencing and DNA barcodes of the Firmicutes and Bacteroidetes 16S rRNA genes to determine the Firmicutes/Bacteroidetes ratio in the gut microbiota of obese humans.
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-10068696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-10964570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-11472932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-14985472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15034147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15333456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15345463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15661851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-15831718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16033867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16056220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16820507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16845028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16845035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16873492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-16880384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17090583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17183309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17183312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17468765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17586664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17686799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17881377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-17916733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18043639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18047683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18248093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18285365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18437230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18665274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18682840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-18818648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19046425-9021275
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
1471-2164
|
pubmed:author |
|
pubmed:issnType |
Electronic
|
pubmed:volume |
9
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
576
|
pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:19046425-Bacterial Typing Techniques,
pubmed-meshheading:19046425-Bacteroidetes,
pubmed-meshheading:19046425-Gastrointestinal Tract,
pubmed-meshheading:19046425-Genes, rRNA,
pubmed-meshheading:19046425-Genetic Markers,
pubmed-meshheading:19046425-Genetic Variation,
pubmed-meshheading:19046425-Humans,
pubmed-meshheading:19046425-Obesity,
pubmed-meshheading:19046425-RNA, Bacterial,
pubmed-meshheading:19046425-RNA, Ribosomal, 16S,
pubmed-meshheading:19046425-Sensitivity and Specificity,
pubmed-meshheading:19046425-Sequence Analysis, DNA
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pubmed:year |
2008
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pubmed:articleTitle |
Use of pyrosequencing and DNA barcodes to monitor variations in Firmicutes and Bacteroidetes communities in the gut microbiota of obese humans.
|
pubmed:affiliation |
URMITE - UMR CNRS 6236, IRD 3R198, Université de la Méditerranée, Faculté de médecine, Marseille, France. fabrice.armougom@univmed.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|