Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-7-17
pubmed:abstractText
Carbonic anhydrase III (CAIII) is an abundant muscle protein characteristic of adult type-1, slow-twitch, muscle fibers. We demonstrate that CAIII is not confined to mature muscle but is also expressed in cultured myogenic cells that were originally derived from adult and fetal limb muscle (G8 and C2C12) and by azacytidine treatment of 10T1/2 fibroblasts (23A2). Transcripts may accumulate in these cells to levels that correspond to 6.5% of that found in mature muscle. CAIII is expressed in mononucleate myoblasts and is abundant in those that preferentially fuse to form myotubes, and these findings contrast with those for many other muscle genes whose transcripts only accumulate on or after terminal differentiation. Preliminary promoter-function assays by transfection shows that 2.8 kb of sequence flanking the 5' end of the human CAIII gene efficiently promotes transcription of the bacterial chloramphenicol acetyltransferase gene in myogenic cells. However, none of the sequences within this region are sufficient to confer muscle-specific expression. Removal of sequences 5' to -715 bp leads to a major loss of transcriptional activity of the CAIII promoter. These results imply that the proximal CAIII promoter, which includes a putative CArG box and four potential MyoD binding sites, is not adequate for either myoblast-specific or maximal transcription.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0740-7750
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:geneSymbol
CAIII
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-28
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1904630-Animals, pubmed-meshheading:1904630-Binding Sites, pubmed-meshheading:1904630-Biological Markers, pubmed-meshheading:1904630-Carbonic Anhydrases, pubmed-meshheading:1904630-Cell Differentiation, pubmed-meshheading:1904630-Cell Line, pubmed-meshheading:1904630-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:1904630-Gene Expression, pubmed-meshheading:1904630-Humans, pubmed-meshheading:1904630-Mice, pubmed-meshheading:1904630-Mice, Inbred C3H, pubmed-meshheading:1904630-Molecular Weight, pubmed-meshheading:1904630-Muscle Proteins, pubmed-meshheading:1904630-Muscles, pubmed-meshheading:1904630-Promoter Regions, Genetic, pubmed-meshheading:1904630-RNA, Messenger, pubmed-meshheading:1904630-Sequence Homology, Nucleic Acid, pubmed-meshheading:1904630-Transcription, Genetic
pubmed:year
1991
pubmed:articleTitle
CAIII a marker for early myogenesis: analysis of expression in cultured myogenic cells.
pubmed:affiliation
MRC Human Biochemical Genetics Unit, Galton Laboratory, London, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't