Linked Life Data

19045842

Source:http://linkedlifedata.com/resource/pubmed/id/19045842

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-12-2
pubmed:abstractText
The authors present the use of electric-field constriction created by a microfabricated structure to realise high-yield electrofusion of biological cells. The method uses an orifice on an electrically insulating wall (orifice plate) whose diameter is as small as that of the cells. Owing to the field constriction created by the orifice, we can induce the controlled magnitude of membrane voltage selectively around the contact point, regardless of the cell size. The field constriction also ensures 1:1 fusion even when more than two cells are forming a chain at the orifice. A device for electrofusion has been made with a standard SU-8 lithography and PDMS molding, and real-time observation of the electrofusion process is made. Experiments using plant protoplasts or mammalian cells show that the process is highly reproducible, and the yield higher than 90% is achieved.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1751-8741
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-9
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
High-yield electrofusion of biological cells based on field tailoring by microfabricated structures.
pubmed:affiliation
Electrical Engineering Department, Chulalongkorn University, Phyathai, Pathumwan, Bangkok 10330, Thailand. boonchai.t@chula.ac.th
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't