|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
2008-12-1
|
|
pubmed:abstractText |
The G alpha subunits of the G(12) family of heterotrimeric guanine nucleotide-binding proteins (G proteins), defined by G alpha(12) and G alpha(13), have many cellular functions in common, including stimulation of stress fiber formation and focal adhesion assembly via the small GTPase RhoA activation. We and others previously showed that G alpha(12) and G alpha(13) mediate neurite retraction in neuronal cell lines, but their roles in primary cultured neurons have not been adequately understood. Here, we found that expression of constitutively active mutants of G alpha(12) or G alpha(13) caused growth cone collapse dependent on Rho-kinase activity in hippocampal neurons. The stimulation of thrombin and lysophosphatidic acid (LPA) receptors, which have been thought to selectively couple to G alpha(12) and G alpha(13), respectively, caused growth cone collapse and suppressed axon branching dependent on Rho-kinase activity in hippocampal neurons. Thrombin- and LPA-induced growth cone collapse was suppressed by both single knockdown of G alpha(12) and G alpha(13) with short hairpin RNAs and this suppression was augmented by double knockdown of both G alpha(12) and G alpha(13). These results suggest that thrombin and LPA receptors couple to both G alpha(12) and G alpha(13) for growth cone collapse.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/CD100 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thrombin,
http://linkedlifedata.com/resource/pubmed/chemical/Semaphorins,
http://linkedlifedata.com/resource/pubmed/chemical/lysophosphatidic acid
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0918-6158
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
31
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2216-22
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:19043202-Animals,
pubmed-meshheading:19043202-Antigens, CD,
pubmed-meshheading:19043202-Axons,
pubmed-meshheading:19043202-Blotting, Western,
pubmed-meshheading:19043202-Cells, Cultured,
pubmed-meshheading:19043202-Cytoplasm,
pubmed-meshheading:19043202-Female,
pubmed-meshheading:19043202-GTP-Binding Protein alpha Subunits, G12-G13,
pubmed-meshheading:19043202-Hippocampus,
pubmed-meshheading:19043202-Indicators and Reagents,
pubmed-meshheading:19043202-Lysophospholipids,
pubmed-meshheading:19043202-Microscopy, Fluorescence,
pubmed-meshheading:19043202-Neurons,
pubmed-meshheading:19043202-Plasmids,
pubmed-meshheading:19043202-Pregnancy,
pubmed-meshheading:19043202-RNA,
pubmed-meshheading:19043202-Rats,
pubmed-meshheading:19043202-Rats, Wistar,
pubmed-meshheading:19043202-Receptors, Thrombin,
pubmed-meshheading:19043202-Semaphorins,
pubmed-meshheading:19043202-Transfection
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Lysophosphatidic acid and thrombin receptors require both G alpha12 and G alpha13 to regulate axonal morphology in hippocampal neurons.
|
|
pubmed:affiliation |
Laboratory of Molecular Neurobiology, Graduate School of Biostudies, Kyoto University, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
