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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-2-23
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pubmed:abstractText |
Ultraviolet (UV) radiation affects the extracellular matrix (ECM) of the human skin. The small leucine-rich repeat protein fibromodulin interacts with type I and II collagen fibrils, thereby affecting ECM assembly. The aim of this study was to evaluate whether short wave UV (UVB) or long wave UV (UVA) irradiation influences fibromodulin expression. Exponentially growing human fibroblasts (IMR-90 cells) were exposed to increasing doses of UVB (2.5-60 mJ/cm(2)) or UVA (0.5-10 J/cm(2)). After UV irradiation fibromodulin, p21 and GADD45 levels were evaluated as well as cell viability, reactive oxygen species formation (ROS) and DNA damage. We found that fibromodulin expression: (i) increased after UVB and UVA irradiation; (ii) was 10-fold higher after UVA (10 J/cm(2)) versus 5-fold with UVB (10 mJ/cm(2)); (iii) correlated with reactive oxygen species formation, particularly after UVA; and (iv) was linked to the DNA damage binding protein (DDB1) translocation in the nucleus, particularly after UVB. These results further suggest that the UV-induced fibromodulin increase could counteract the UV-induced connective tissue damage, promoting the assembly of new collagen fibrils.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GADD45A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Trypan Blue,
http://linkedlifedata.com/resource/pubmed/chemical/fibromodulin
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1638-6183
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
364-72
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pubmed:meshHeading |
pubmed-meshheading:19041686-Cell Cycle Proteins,
pubmed-meshheading:19041686-Cell Survival,
pubmed-meshheading:19041686-Cells, Cultured,
pubmed-meshheading:19041686-Coloring Agents,
pubmed-meshheading:19041686-Comet Assay,
pubmed-meshheading:19041686-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:19041686-DNA Damage,
pubmed-meshheading:19041686-DNA-Binding Proteins,
pubmed-meshheading:19041686-Dose-Response Relationship, Radiation,
pubmed-meshheading:19041686-Extracellular Matrix Proteins,
pubmed-meshheading:19041686-Fibroblasts,
pubmed-meshheading:19041686-Gene Expression Regulation,
pubmed-meshheading:19041686-Humans,
pubmed-meshheading:19041686-Nuclear Proteins,
pubmed-meshheading:19041686-Proteoglycans,
pubmed-meshheading:19041686-RNA, Messenger,
pubmed-meshheading:19041686-Reactive Oxygen Species,
pubmed-meshheading:19041686-Trypan Blue,
pubmed-meshheading:19041686-Ultraviolet Rays
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pubmed:year |
2009
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pubmed:articleTitle |
Ultraviolet B and A irradiation induces fibromodulin expression in human fibroblasts in vitro.
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pubmed:affiliation |
Department of Biochemistry, Faculty of Biotechnology, University of Naples Federico II, via S. Pansini 5, 80131 Naples, Italy.
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pubmed:publicationType |
Journal Article
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